摘要
以10mmol/L CaC l2溶液处理花生幼苗叶片后,置于培养箱42℃高温培养,定时测定幼苗叶叶绿体Ca2+-ATPase,Mg2+-ATPase活性,并观察幼叶细胞内Ca2+分布的变化。试验结果表明,外源Ca2+处理相应提高和保护了Ca2+-ATPase,Mg2+-ATPase的活性;外源Ca2+预处理能够明显增加细胞间隙、细胞壁、质膜和叶绿体上Ca2+颗粒密度;高温胁迫后,Ca2+具有从胞外转运到胞质内和叶绿体中的趋势;Ca2+能够稳定高温胁迫下叶肉细胞膜和叶绿体的超微结构。
Peanut seedlings sprayed with 10mmol/L CaCl2 were cultured at 42℃, and the activities of Ca^2＋-ATPase、Mg^2＋-ATPase in chloroplast were measured regularly and Ca^2＋ distribution in young leafage was observed timely. The results showed that Ca^2＋ could evidently improve and protect the activities of Ca^2＋-ATPase、Mg^2＋-ATPase, and Ca^2＋ pretreatment could induce changes of Ca^2＋ distribution and significantly increase the Ca^2＋ level in intercellular spaces, vacuoles and chloroplasts. When calcium-treated leaves were under heat stress at 42℃ , the amount of Ca^2＋ located in intercellular space decreased, and most of them transferred to cytosol and chloroplast across the plasmalemma. The results suggest that exogenous Ca^2＋ results in the stabilization of membrane and chloroplast structures under high temperature stress.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2005年第4期41-44,49,共5页
Chinese Journal of Oil Crop Sciences
基金
江苏省自然科学基金(1998SWXOZ001)
