摘要
目的研究人端粒酶逆转录亚单位(hTERT)特异性siRNA对MCF-7细胞体外生长及体内肿瘤形成能力的抑制作用。方法设计并化学合成针对hTERT的siRNA,用脂质体转染法将其导入MCF-7细胞内,通过软琼脂克隆形成试验及接种裸鼠的肿瘤形成实验,观察hTERT-siRNA对人乳腺癌MCF-7细胞体外及体内的生长抑制作用。结果软琼脂克隆形成试验显示,hTERT-siRNA转染的MCF-7细胞克隆形成数量明显少于对照组,抑制率达到84.1%。裸鼠体内实验结果显示,hTERT-siRNA转染组肿瘤生长速度也明显慢于对照组。结论hTERT-siRNA在体内外均显示可以有效、特异地抑制肿瘤细胞的生长。
Objective The abnormal high expression of telomerase is closely correlated with the initiate, development and prognosis of cancer. The telomerase catalytic subunit（hTERT） is a speed-limited factor of telomerase synthesis. The aim was to study inhibition of siRNA against hTERT gene on breast cancer cells（MCF-7）. Methods The siRNA synthesized chemically was transfected into the MCF-7 cells with lipofectamine TM2000. Then using soft agar colony forming assay and in SCID nude xenograft of cancer,we investigated the tumorigenesis ability of the MCF-7 cell tranfected with hTERT-siRNA in vitro and in vivo. Results Soft agar colony forming assay showed that the colony number of hTERT-siRNA group was significantly lower than that of the other two control groups. The inhibition rate in hTERT-siRNA group was 84.1%. The experiment in vivo showed that the tumor in hTERT-siRNA group grew more slowly than the control groups. Conclusion The hTERT-siRNA can inhibit the growth ability of the MCF-7 cells in vitro and in vivo.
出处
《实用癌症杂志》
2005年第5期452-454,461,共4页
The Practical Journal of Cancer
基金
天津市科委重点攻关项目
项目编号:0233111511-8
