摘要
从食草动物的粪便中经筛选分离到1株能降解纤维素的放线菌,经初步鉴定为Streptomycesspp.。对其在以秸秆为惟一碳源的培养基上的产酶条件进行研究,结果表明,最适摇瓶发酵产酶条件为以硫酸铵为氮源,采用种龄72 h的菌液接种,在接种量10%、培养基装量1/10、培养温度30℃时,发酵60 h CMCase活力可达4.5 u/mL。
A cellulose-decomposing Actinomyces XW5 strain was isolated from dejection of herbivore by repeated screening. It was found that it belongs to Streptomyces. Its enzyme-producing conditions with crop stalk as unique carbon source was studied. The results showed that the optimum enzyme-producing conditions were （NH4）SO4 as nitrogen source, inoculum age at 72 h, and 10% of inoculation, medium volume 1/10, 30℃ , and shaking at 120 r/ min. The enzyme activity reached 4.5 u/mL after 60 hour of culture.
出处
《微生物学杂志》
CAS
CSCD
2005年第5期36-39,共4页
Journal of Microbiology
基金
西华师范大学引进优秀人才基金(2003[6])
关键词
链霉菌属放线菌XW5
纤维素降解
筛选
纤维素酶
Streptomyces
cellulose-decomposing
screening
cellulase
fermentation condition
