摘要
目的探讨胃组织中基质金属蛋白酶-2(MMP-2)和黏附分子CD44拼接变异体V6(CD44v6)基因的表达与胃癌生物学行为的相关性。方法应用免疫组化S-P法,对40例胃癌手术切除标本进行抗人MMP-2和CD44v6单克隆抗体免疫组化染色,检测癌组织、癌旁组织、手术切缘区正常组织各区域MMP-2和CD44v6蛋白表达,并分析二者之间及二者的表达与胃癌临床病理特征之间的关系。结果MMP-2蛋白表达在癌组织与手术切缘区正常组织、癌组织与癌旁组织之间差异有显著性,癌旁组织与手术切缘区正常组织之间其表达差异无显著性;CD44v6蛋白表达在癌组织与手术切缘区正常组织、癌组织与癌旁组织、癌旁组织与手术切缘区正常组织之间差异均具有显著性。癌组织中MMP-2和CD44v6蛋白表达与患者性别和年龄无相关性;而与肿瘤分化程度、浸润深度、淋巴结转移、临床TNM分期呈正相关;CD44v6蛋白表达还与肿瘤有无远处转移有关。此两种蛋白表达彼此具有相关性,即同时表达阳性的胃癌病例具有更为恶性的生物学特征。结论MMP-2和CD44v6在胃癌的发生和侵袭转移中可能起着重要作用,检测MMP-2和CD44v6的表达有望成为判断胃癌病变发展、预测肿瘤转移潜能的客观指标。
Objective To study the relationship between the expression of MMP-2, CD44v6 and the biological behavior of gastric cancer. Methods A total of 40 specimens resected was investigated by S-P im- munohistochemical staining with an anti-MMP-2 monoclonal antibody and an anti-CD44v6 monoclonal anti- body. The expression of MMP-2 and CD44v6 in various regions of the gastric cancer tissues, paracancerous tissues and incision margin normal tissues were detected and the relationship with clinicopathologic factors was analyzed. Results The difference of MMP-2 expression in gastric cancer tissues versus incision margin normal tissues and gastric cancer tissues versus paraeancerous tissues was statistically significant, but there was no difference of MMP-2 expression in paracancerous tissues versus incision margin normal tissues. The difference of CD44v6 expression in gastric cancer tissues versus incision margin normal tissues, gastric cancer tissues versus paraeancerous tissues,and paracancerous tissues versus incision margin normal tissues was statistically significant. The expression of MMP-2 and CD44v6 protein in gastric cancer tissues was not related to sex and age, but was positively related to the differentiation, depth of invasion, lymph node metastasis, and TNM stage,and the expression of CD44v6 protein was related to distant metastasis of carcinoma. There was a positive correlation between the MMP-2 expression and the CD44v6 expression. Conclusion MMP-2 and CD44v6 may play an important role in the carcinogenesis, invasion and metastasis of gastric carcinoma, expression of MMP-2 and CD44v6 might serve as an objective indicator for estimating tumor progress and predicting occurrence of cancer cell metastasis.
出处
《中国肿瘤临床与康复》
2005年第6期502-506,共5页
Chinese Journal of Clinical Oncology and Rehabilitation
