摘要
目的:建立以高效液相色谱法测定妇炎康浓缩丸中苦参碱含量的方法。方法:色谱柱填充剂为氨基键合硅胶,流动相为乙腈-无水乙醇-3%磷酸溶液(80∶10∶10),检测波长为220nm。结果:苦参碱进样量在0·4064μg^2·0320μg范围内与峰面积积分值线性关系良好(r=1·0000),平均回收率为99·2%(RSD=1·6%)。结论:本方法较薄层色谱法准确度高,可用于本品的质量控制。
OBJECTIVE：To determine the content of sophocarpidine in Fuyankang concentrated pill by HPLC. METHODS： The loading agent of chromatographic column was amino bonded silica, the mobile phase was acetonitrile- dehydrated alcohol- 3%phosphoric acid （80 ： 10 ： 10）, and the detection wavelength was 220nm.RESULTS： There was a good linear relationship between sample size and peak area score of sophocarpidine in the range of 0.4 064μg-2.0 320μg, the average recovery rate was 99.2% （RSD- 1.6% ）. CONCLUSION：The established method is more accurate than TLC, and it can be used for the quality control of Fuyankang concentrated pill.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第24期1901-1902,共2页
China Pharmacy
关键词
高效液相色谱法
妇炎康浓缩丸
苦参碱
含量
High performance liquid chromatography
Fuyankang concentrated pill
Sophocarpidine
Content
