摘要
目的:应用热休克人胶质瘤细胞的方法,观察热诱导热休克蛋白70(heatshockprotein70,HSP70)在人胶质瘤细胞的表达。方法:经43℃热处理2h的人胶质瘤细胞,应用半定量逆转录聚合酶链反应(RTPCR)及免疫组化技术检测HSP70mRNA及蛋白的表达情况。结果:热休克蛋白人胶质瘤细胞表达阳性率为(39.40±14.40)%,对照组为(8.50±4.60)%及HSP70mRNA表达光密度值为0.23±0.05,对照组为0.13±0.03与内参照βactin比值经单因素方差分析q检验显示与对照组相比有明显的升高。结论:热休克的方法可以明显诱导人胶质瘤细胞HSP70的表达。
OBJECTIVE： To investigate the expression of HSP70 in human glioma cells by means of heat shock human glioma cells. METHODS： Human glioma cells were cultured in vitro in the condition of 37℃ for 2 hours. The protein of HSP70 was examined by means of immunochemistry and the level of mRNA was measured by RT-PCR method. RESULTS： The positine rate of HSP70 expression were （39.40± 14.40）% and （8.50± 4.60） ^ in the treatment group and the contronl group, the optical density number of HSP70 mRNA were 0.23±0.05 and 0. 13 ±0.03 in the treatment group and the contronl group, they and inner consult β-actin ratio were abaktsed bt ANOVA q TEST respeceively. It showed that there were significant enhanceneats compared with the control group. CONCLUSION： The heat shock can induce the expression of HSP70.
出处
《肿瘤防治杂志》
2005年第21期1606-1608,共3页
China Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30030781)
