摘要
目的观察层黏连蛋白(LN)在小鼠肾发育过程中的表达规律及层黏连蛋白与细胞极性的关系。方法用免疫组织化学链霉菌素亲和生物素-过氧化物酶连接法测定不同胚龄和日龄小鼠肾层黏连蛋白的表达。结果层黏连蛋白在肾组织中的表达随着胚龄增大和肾成熟程度而增加。在生后肾组织中的单个细胞、多个细胞无腔形成和多个细胞有腔形成时均有表达。在肾发育过程中层黏连蛋白表达于输尿管芽周围、肾小体基膜、肾小管基膜、肾小囊基膜和肾间质,且肾小管基膜着色早于肾小体基膜。结论在肾发生和成熟过程中,层黏连蛋白参与细胞极性的形成,层黏连蛋白的分布具有一定的时空顺序,对基膜的发生、成熟起重要作用。
Objective To observe whether laminin （LN） is expressed in the developing kidney of mouse and the changes of the expression associated with maturation and polarity. Methods Eight fetal and fourteen postnatal kidneys were examined by immunohistochemistry （Strepavidin Peroxidase conjugated method, SP）, using polyclonal rabbit anti-mouse antibody. Results The expression of laminin in the kidneys increased with the gestational weeks of fetus and maturity of the kidney. The expression of laminin was positive in monocell, muhicell without space and multicell with space. In the developing kidney, the laminin is expressed in the ureteric bud, glomerular basement membrane （ GBM ）, tubular basement membrane （ TBM ）, renal capsular basement membrane and interstitium. The expression of TBM was prior to the expression of GBM. Conclusion The expression of laminin in the developing kidney of mouse has chronological and spatial sequence. The laminin determines the polarity of cell. Laminin might play a key role during nephrogenesis.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第6期688-691,共4页
Acta Anatomica Sinica
基金
辽宁省自然科学基金项目(20032140)
