摘要
目的观察大鼠中央杏仁核内微量注射P物质(SP)对胃肌电和胃运动幅度的影响。方法用铂金丝双电极和应变片传感器引导胃肌电及胃运动信号,用MacLab系统记录并处理信号。结果中央杏仁核内注射4 g/L的SP1μL,可明显抑制胃电-机械活动;注射1.5 g/L的SP受体拮抗剂(DPDPDT)1μL,可阻断内源性SP的作用,胃电-机械活动增强;并且DPDPDT可使外源性SP对胃电-机械活动的抑制效应减弱;用0.5 g/L阿托品1μL阻断M受体后,SP对胃电-机械活动的抑制效应也减弱。结论SP在中央杏仁核可能通过与SP受体结合,抑制胃电-机械活动,而胆碱能神经元可能参与了SP抑制胃电-机械活动这一过程。
Objective To provide experimental evidences that SP can modulate the functionality dyspepsia caused by long term emotional tension. Methods Bipolar electrodes and high sensitive stress plate transducer were used to educe gastric myoelectric activity and mechanical movement in gastric antrum. MacLab system was used to record and process the signals. Results The results showed that microinjection of 1 μL SP （4 g/L） into the central amygdaloid nucleus can significantly inhibit gastric myoelectric activity and mechanical movement. Microinjection of 1 μL SP receptor antagonist [D-Pro2, D-Phe7, D-Trp9] -Substance P （DPDPDT） （1.5 g/L） can block the action of endogenous SP and augment gastric electro-mechanical activity. At the same time, DPDPDT can weaken the inhibitory effects of exogenous SP on gastric myoelectric activity and mechanical movement. Microinjection of 0.1 μL Atropine （0.5 g/L）, a kind of M receptor blockers, can also weaken the inhibitory effects of SP on gastric myoelectric activity and mechanical movement. Conclusion These results indicate that SP exerts inhibitory effects on regulation of gastrointestinal electro-mechanical activity by binding to SP receptor in central amygdaloid nucleus. Cholinergic neuron may play a role during this process.
出处
《基础医学与临床》
CSCD
北大核心
2005年第12期1165-1169,共5页
Basic and Clinical Medicine
关键词
胃电-机械活动
P物质
P物质受体拮抗剂
中央杏仁核
大鼠
gastric electro-mechanical activity
substance P
substance P receptor antagonist
central amygdaloid nucleus
