摘要
从土壤中分离到对黄瓜枯萎病病原菌有抑制作用的枯草芽孢杆菌(Bacillus subtilis),发酵产物经DEAE-纤维素、Sephadex G-100柱层析,分离到15 kD抑菌蛋白L37,SDS-PAGE显示纯度达到电泳纯,N-末端氨基酸序列同源性检测结果提示可能是一种新型的抑菌蛋白。蛋白质抑菌结果表明,对多种病原真菌具有抑制效果,且对蛋白酶、温度和大部分有机物不敏感,是稳定的抑菌蛋白。对病原菌菌丝抑制机理研究表明,抑菌蛋白具有严重扭曲、断裂、阻止生长等抗生效果。应用发酵蛋白产物进行两年大田防效试验表明,对黄瓜枯萎病防效显著,最高达到90%,具有显著提高产量的作用。
Bacillus subtilis inhibiting cucumber wither pathogen was screened from soil. After fermenting, 15 kD antagonistic protein named L37 was got through DEAE-fibrin, Sephadex G-100 chomatography, which proved purified completely through SDS-PAGE. It was probably a new type of antagonistic protein against pathogen by the sequence on the amino N-terminal testing, which could make the normal hypha of pathogen distort, split and inhibit. The control effect could reach 90% against cucumber wither pathogen and improved the yield in field controlling test by fermenting products.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第6期1102-1104,共3页
Acta Horticulturae Sinica
基金
国家‘863’项目(2002AA212151)
四川‘十五’科技攻关项目
关键词
黄瓜
黄瓜枯萎病
枯草芽孢杆菌
抑菌蛋白
防治
Cucumber
Cucumber wither
Bacillus subtilis
Antagonistic protein
Controlling
