摘要
目的了解结缔组织生长因子(CTGF)是否诱导肾小球系膜细胞产生趋化因子RANTES,了解脂氧素A4(LXA4)是否影响CTGF对合成RANTES的诱导作用,并探讨其作用机制。方法应用CTGF刺激培养的大鼠肾小球系膜细胞,应用RT-PCR方法测定RANTES的mRNA表达,应用ELISA测定上清液中RANTES。应用趋化试验测定上清液对单核细胞(THP21)的趋化作用。应用Westernblot测定分裂原激活的蛋白激酶(p42/44MAPK)、磷脂酰肌醇32激酶(PI32K)和蛋白激酶B(PKB)的表达。应用凝胶电泳迁移率试验测定核因子2κB(NF2κB)的表达。构建含脂氧素A4受体同源基因(LRHG)的真核表达载体pcDNA3.1PLRHG,并转染系膜细胞,观察LXA4对CTGF作用的调节是否依赖LRHG。结果CTGF刺激使系膜细胞RANTES的mRNA表达与分泌量增加,增加磷酸化(P)-p42P44MAPK、P2PI32K、P2PKB及NF2κB表达。LXA4呈剂量依赖性地抑制CTGF所致的上述变化。P2p42/44MAPK抑制剂PD98059抑制CTGF诱导的p42/44MAPK磷酸化与RANTES分泌。PI32K抑制剂LY294002抑制CTGF诱导的PI32K、PKB、NF2κB活化与RANTES分泌。NF2κB抑制剂PDTC抑制CTGF诱导的NF2κB活化与RANTES分泌。pcDNA3.1PLRHG转染使LXA4对CTGF诱导的p42/44MAPK磷酸化与RANTES的分泌的抑制作用增强。结论LXA4可抑制CTGF引起的系膜细胞分泌RANTES,其机制依赖于抑制p42/44 MAPK、PI32K/PKB的磷酸化与NF2κB活化。而LRHG转染细胞可增强LXA4的抑制作用,提示LRHG参与了LXA4的抑制作用。
Objective To determine the regulatory role of connective tissue growth factor （CTGF） on the production of RANTES in rat glomerular mesangial cells, and the modulatory effect of lipoxin A4 （ LXA4 ） on the action of CleF, and to explore the mechanisms of action of CleF and LXA4. Methods Cultured rat mesangial cells were treated with CTGF with or without preincubafion with LXA4. Expression of mRNA was analyzed by RTPCR. Protein of RANTES in the supematants was determined by ELISA. Monocyte transmigration was assessed by in vitro chemotaxis assay. Expression of p42/44 mitogen-activated protein kinase（ MAPK）, phosphoinosifide 3-kinase（PI3-K） and protein kinase B（PKB） were assessed by Western blot. Activity of nuclear factor-kB（NF-xB） was determined by electrophroretic mobility shift assay（EMSA）. To observe whether transfection of LXA4 receptor hoinologue gene（LRHG） into mesangial cells intensified these modulatory effects of LXA4, mesangial cells were transfected with pcDNA3.1/LRHG vector. Results CTGF enhanced the mRNA expression and protein release of RANTES, the expression of phospho（P）-p42/44 MAPK, P-PI3-K, P-PKB and NF-xB. P-p42/44 MAPK blockade inhibited the CTGF-induced expression of P-p42/44 MAPK and partially decreased the level of RANTES in supernatants. P-PI3-K blockade down regulated the CTGF-stimulated expression of P-PI3-K, P-PKB and NF-kB, and partially decreased the release of RANTES. NF-kB blockade abrogated the CTGF-activated NF-kB and partially decreased the secretion of RANTES. LXA4 dose-dependently inhibited the CTGF-sfimulated above action. Transfection of LRHG into mesangial cells intensified these inhibitory effects of LXA4 on CTGF-induced release of RANTES and expression of the P-p42/44 MAPK. Conclusion LXA4 inhibits CTGF-induced production of RANTES via p42/44 MAPK, PI3-K/PKB and NF-kB-dependent signal pathway, that is mediated by LRHG in rat mesangial cells.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2005年第11期901-906,共6页
Chinese Journal of Microbiology and Immunology
基金
江苏省135医学重点人才工程基金(2002245)
