期刊文献+

转解毒酶基因工程菌的构建及酶活性分析

Construction of Detoxifying Gene Recombinant Bacterium and Enzyme Activity Analysis
下载PDF
导出
摘要 实验构建了能高效降解有机磷、有机氯等四大类农药的转解毒酶基因工程菌。将抗性库蚊解毒酶酯酶B1基因片段引入融合表达载体pThioHisA中,转化入大肠杆菌DH5α,在IPTG诱导下,经过8 h,酯酶B1在大肠杆菌中的获得融合高效表达。研究了工程菌的酶酯活性,重组质粒pThioHisA-B1表达的酯酶融合蛋白具有较高的酯酶B1活性,能高效降解酯酶的特异性底物α-乙酸萘酯(-αNA)和β-乙酸萘酯(-βNA),该工程菌将为农药污染的生物治理提供新手段。 A detoxifying gene esterase B1 was cloned into fusion expression vector pThioHisA. The recombinant vector pThioHisA-B1 was constructed and transformed into E. coli DH5a. High level of expression of the target protein was obtained 8 hours after induced by IPTG. The protein expressed by pThioHisA-B1 possessed high esterase activity and it can degrade the specific substrate α-NA and β-NA of esterase effectively. A novel engineering bacterium containing recombinant esterase B1 gene was constructed that can degrade four kinds of insecticides including organophosphate and organochlorine. The use of the engineered bacterium could provide a novel way in bioremediation of pesticide contamination.
出处 《微生物学杂志》 CAS CSCD 2005年第6期45-48,共4页 Journal of Microbiology
关键词 酯酶基因 融合表达 工程菌 酶活性 esterase gene fusion expression engineered bacterium enzyme activity
  • 相关文献

参考文献15

二级参考文献66

共引文献71

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部