摘要
对双相氯胺-T碘标法实验装置作了改进并标记长效促红细胞生成素(LL-EPO),采用离心超滤技术代替传统的柱层析脱盐纯化标记蛋白质。结果显示125I-LL-EPO具有高比活性、高生物活性、高回收率。说明双相氯胺-T碘标法简单方便,是蛋白质同位素碘标记的理想方法;离心超滤技术适合放射性碘标记蛋白质的纯化。
This research presented a modified two-phase chloramine- T method for radioiodine labeling of protein and Long-lasting Erythropoietin （LL-EPO） was labeled. And traditional gd filtration was substituted by ultrafiltration to purify the labeled protein. The results show that labeled protein possesses high specific radioactivity, biological activity and recovery. It suggests that two-phase chlo- famine-T method is simple and convenient and an ideal method to iodinate protein. Ultrafiltration is suitable for purification of the radioiodine labeled protein.
出处
《四川农业大学学报》
CSCD
2005年第4期428-431,共4页
Journal of Sichuan Agricultural University
