摘要
目的利用流式细胞术(FCM)检测不同患者T淋巴细胞表面HLA-B27抗原,与传统的血清法(微量淋巴细胞毒试验)比较,评价FCM检测HLA-B27在强直性脊柱炎(AS)诊断中的应用价值。方法采用荧光标记的HLA-B27单抗与T淋巴细胞表面的HLA-B27抗原结合,利用FCM专门分析软件测定HLA-B27-FITC平均荧光强度(FMI),从而间接定量HLA-B27表达情况,并采用血清法对118例AS患者标本进行HLA-B27检测。结果AS患者HLA-B27阳性率为88.9%(105/118),男女比例为5.25∶1,同其他各组比较均有较显著的差异(P<0.01);两种方法比较无明显差异(P>0.1)。结论利用FCM对疑为AS的患者进行HLA-B27抗原检测有助于AS的诊断,方法简便,稳定性好,重复性高,是目前较为理想的方法。
Objective To investigate the application of thymus lymphocyte antigen HLA-B27 expression detection with flow cytometer （FCM） in the diagnosis of ankylosing spondylitis （AS） by comparing with traditional microcytotoxicity assay method. Methods The fluorescence labeling monoclonal antiboly of HLA-B27 was used to conjugate with thymus lymphocyte antigen HLA- B27. The expression of antigen HLA - B27 was quantitatively measured indirectly by determining the fluorescence mean intensity （ FMI ） of HLA- B27 - FITC measured with professional analysis software of FCM, While the samples of 118 cases with AS were measured with traditional microcytotoxicity assay method. Results The positive incidence of antigen HLA-1327 was 88, 9% （105/118） in AS patients, and male vs female was 5,25：1. There was significantly difference between the cases with AS and the cases with pains of the lower back and legs or individuals accepting physical examination （ P〈0.01 ）, but the difference between the two methods was not significant （ 0.1 〈 P 〈 0.25 ）, Conclusions The detection of antigen HLA - 1327 expression with FCM is helpful to the confirmed diagnosis of AS, FCM is an easy,stable,accurate detection method with higher repeatability,
出处
《实用全科医学》
2006年第1期96-97,共2页
Applied Journal Of General Practice
