摘要
本试验为研究不同运输温度和体外成熟时间对卵母细胞的影响,从屠宰场共采集894枚屠宰绵羊卵母细胞,分两批处理,一批分别处于10～15℃,20～25℃, 30～35℃三种不同的运输温度;另一批通过16～18h,20～22h,24～26h,28～30h四种不同的体外成熟时间,后经体外受精观察其发育情况.结果发现:①在不同运输温度的卵母细胞中,温度为20～25℃时卵母细胞能够进行最好的发育,达到第一极体的卵母细胞为64.86%,达囊胚的卵母细胞为34.58%.②在不同体外成熟时间的卵母细胞中,时间为20～22h时卵母细胞能够最好的发育,达到第一极体的卵母细胞为62.79%,达囊胚卵母细胞为31.70%.
In order to find the optimal transfer temperature to carry ovaries and the optimal length of in vitro maturation, ovaries were transferred under different temperatures ： 10 - 15℃, 20 - 25℃, 30 - 35℃. Collected ooeytes were matured for different length of time： 16 - 18h,20 -22h,24 - 26h,28 - 30h. The result shows ： the optimal transfer temperature of overies is 20 - 25℃ . Because oocytes collected from ovaries transferred under 20 - 25℃ had the highest rate （64.86%） to be fertilized . Optimal length of in vitro maturation is 20 -22h, because oocytes had the highest rate （62.79%） to be fertilized .
出处
《塔里木大学学报》
2005年第4期5-7,共3页
Journal of Tarim University
关键词
绵羊
卵母细胞
温度
体外成熟时间
体外受精
sheep oocyte
length of in vitro maturation
in vitro- fertilization
temperature
