摘要
用人Ⅳ型胶原蛋白免疫ＢＡＬＢ／ｃ小鼠，克隆了一组分泌抗人Ⅳ型胶原蛋白的杂交瘤细胞株，并且对其中的Ａ２、Ａ４、Ａ５、Ｂ３和Ｅ２进行了鉴定。其抗体亚类分别为ＩｇＧ１、ＩｇＧ２、ＩｇＧ２ｂ、ＩｇＧ２和ＩｇＧ１。其亲和力常数Ｋａｆｆ＝６．６×１０８Ｍ（－１）～６．０×１０（１０）Ｍ（－１）。它们均能特异性地识别人Ⅳ型胶原蛋白。
Hybridomas secreting McAbs to human collagen type Ⅳ were established by immunization of BALB/c mice with human collagen type Ⅳ (Ⅳ-CL). Five of these McAbs A2, A4,A5, B3 and E2 were well characterized and the Ig class and subclass were determined as IgG1,IgG2, IgG2b, IgG2 and IgG1 respectively. These McAbs reacted strongly with Ⅳ-CL moleculeswith the affinity constants(Kaff) of 6.6×108M-1～6.0×1010M-1 and did not have cross-reactivity with other types of collagen and irrelevant proteins.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第2期1-3,共3页
Chinese Journal of Cellular and Molecular Immunology
