摘要
通过对驱蚊草离体叶片和茎段的培养及植株再生的研究,成功地建立了驱蚊草组织培养快繁技术体系.用0.1%升汞对叶片、叶柄和茎段进行消毒,最佳消毒时间分别为6.5、6.0、7.0 m in;叶片和茎段不定芽诱导的最适培养基为M S+BA(1.0 m g/L)+NAA(1.0 m g/L),叶柄为M S+BA(0.5 m g/L)+NAA(0.5 m g/L);不定芽的最适增殖培养基为M S+BA(0.75 m g/L)+NAA(0.6 m g/L)+GA3(0.2 m g/L),增殖倍数为6.1;最适生根培养基为1/2 M S培养基,生根率92%,平均每株生根数为10条.
By studying the culture of the detached leaves and stems and the regeneration of Mozzie buster plantlets, the experimental-system of rapid propagation of Mozzie buster in vitro have been successfully established. With 0.1% HgCl2, the optimum sterilization times for leaves, petioles and stems are 6.5, 6.0 , 7.0 min respectively. The most suitable medium for the initiation of adventitious buds from leaves and stems is MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L NAA, and that from petioles is MS medium supplemented with 0.5 mg/L BA and 0.5 mg/L NAA. The best medium for the propagation of adventitious buds is MS medium supplemented with 0. 75 mg/L BA, 0. 6 mg/L NAA and 0.2 mg/L GA3. The ratio of buds propagation is 6.1, 1/2 MS medium is most suitable for the rooting of shoots. The rate of rooted shoots is 92% and the average number of roots per shoot is 10.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第1期72-76,共5页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省教育厅基金资助项目(JB02162)
关键词
驱蚊草
组织培养
再生体系
Mozzie buster
tissue culture
regeneration system
