摘要
目的建立高效液相色谱测定强力痔根断片中芦丁含量的方法。方法选用C18色谱柱,甲醇-水-冰醋酸(40∶60∶1.8)为流动相,检测波长为354nm,柱温30℃,结果芦丁在6.008～48.064μg/mL浓度范围内与峰面积呈良好的线性关系,(r=0.9999),平均回收率100.3%,RSD=0.5%(n=5)。结论该方法简便、快速、分析结果准确、稳定,专属性强,可用于强力痔根断片的质量控制。
Objective To determine the content of rutin in Circanetten New tablets by HPLC. Methods The determination is performed at C18（150 mm × 4.6 mm, 5 μm） column with temperature being 30℃; the mobile phase is consisted of methanol- water- glacial acetic acid（40 ： 60 ： 1.8） and UV detection wavelength is at 354 nm. Results The linear range was 6. 008 to 48. 064μg/mL （ r =0. 999 9） for Circanetten New tablets and the average recovery of rutin was 100. 3% （RSD = 0.5 % , n = 5） . Conclusion The method is simple and effective, and can be used to control the quality of Circanetten New tablets.
出处
《中药新药与临床药理》
CAS
CSCD
2006年第1期52-54,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
