摘要
用一对根据人线粒体细胞色素ｂ基因保守区序列设计的引物，以一枚恐龙蛋内ＤＮＡ为模板，在ＰＣＲ上扩增出大约１７０ｂｐ的基因片段。序列测定后经ＩＮＴＥＲＮＥＴ联网的ＢＬＡＳＴ数据库类似性检索，结果表明，该序列与人类线粒体细胞色素ｂ基因的序列完全相同（９６－１００％相似）。因此，可以肯定实验材料本身有严重污染。
A 170bp PCR products was amplified the DNA in a dinosaur egg using primers designed from the conserved regions of human cytochrome b gene and was sequenced.BLAST search through INTERNET for homology detection showed that the sequence was almose identical with the human mitochondria cytochrome b gene(96-100% similarity).Therefore we can conclude that the dinosaur egg itself has been seriously contaminated.
关键词
恐龙
恐龙蛋
细胞色素B
基因序列分析
Dinosaur egg,Sequencing of cytochrome b gene,Contamination
