摘要
本文研究了以重氮化的对—氨基苯磺酰乙基纤维素为载体制各固定化果胶酶的最适条件，并比较了固定化果胶酶与游离酶的性质。结果表明，最适的固定化果胶酶的条件是：在ｐＨ７．００．１５Ｍ的磷酸盐缓冲液中，按每克载体加入２１６３活力单位的酶的比例进行偶联反应１２小时。在以上最适条件下，固定化果胶酶的表观活力为１９８０Ｕ／ｇ，活力回收率为８７％。与游离酶相比，固定化果过酶作用的最适ｐＨ由４．６移至４．２，最适温度变宽，酶的热稳定性增强，操作稳定性良好，半衰期为３２．５天。
In this paper,some factors that affect immobilization of pectinase on diazotized p aminobenzene sulphonyl ethyl cellulose were studied and some properties of the immobilized pectinase reported. Results showed that the optimum conditions for coupling reaction were: 2163units of pectinase should be coupled with lg diazotied p-aminobenzene sulphonyl ethyl cellulose,coupling time should be 12 hours,the PH and concentration of sodium phosphate buffer should be 7.0 and 0.ibM respectively.Under optimum conditions for the coupling, the apparent activity and activity recovery of immobllized pectinase were 1980u/g and 87 % respectively. The optimum pH of the immobilized pectinase shifted from 4. 6 to 4.2.and its optimum temperature became rather broad. The thermostability was much higher than that of the native enzyme. The immobilized pectinase had good operational stability and half-life of 32. 5 days.
出处
《生物技术》
CAS
CSCD
1996年第2期26-29,共4页
Biotechnology
关键词
果胶酶
固定化
Pectinase
Immobilization
