摘要
提出一种制作高密度DNA微阵列的非接触式点样新方法。压电陶瓷低频微振动使得微流体产生均匀的离散和喷射;对微喷嘴内壁进行疏水化处理,从而消除微流体由于湿润作用在微喷嘴内部的自发性流动,使得微喷射具有数字化量可控的特征。通过微流体粘度控制、微喷射速度和微喷射距离的控制消除样点缺陷,获得了均匀的点样样点。通过微喷嘴几何尺寸控制和驱动频率控制获得从飞升到皮升级样点体积,可以用于制作高密度的微阵列。惯性力在微米尺度下仍然起主导作用,可以驱动微流体中微粒的移动,消除微喷嘴堵塞现象。
A kind of new method for fabricating non-contact styled spotting of high density DNA micro-array was put forward. Low frequency micro-vibration causes the micro-fluid to produce uniform dispersion and injection which carried out a hydrophobization treatment on the inner wall of micro-nozzle, thus eliminated the spontaneous flowing of micro-fluid inside the micro-nozzle due to wetting effect so as to let the micro-injection be provided with a digitalized and volume controllable character. The uniform spotting droplets were obtained by means of the controls of micro-fluid viscosity, micro-injection speed and micro-injection distance for eliminating the defects of droplet. Through the controls of geometric dimension of micro-nozzle and driving frequency to obtain the volume of droplets in the grade of pico to femto litre, which is applicable to make high density micro-array. Inertial force still plays leading role under micro-metre scale, it could move the particles in micro-fluid so as to eliminate the chocking phe nomenon of micro-nozzle.
出处
《机械设计》
CSCD
北大核心
2006年第1期6-10,共5页
Journal of Machine Design
基金
国家自然科学基金资助项目(50275079)
博士学科点专项科研基金资助项目(2001028813)
关键词
微流体
离散化
微喷射
微阵列
点样
micro-fluid
discretization
micro-injection
micro-array
spotting
