摘要
目的:建立以紫外分光光度法测定西地那非电离平衡常数的方法。方法:将pH值各为2.26、8.56、9.31、9.83、12.38的西地那非甲醇溶液分别于200nm～400nm波长范围内扫描,并在选定的280、287、290、295、297nm波长处测定其吸收度,计算电离平衡常数的负对数(pKa)值。结果:西地那非的pKa值为9.35±0.02。结论:本方法简便、易行。
OBJECTIVE：To establish a method for determination of ionization equilibrium constant of sildenafil using ultraviolet spectrophotometry. METHODS： Methanol solutions of sildenafil with pH values of 2.26, 8.56, 9.31, 9.83 and 12.38 were respectively scanned at the wavelength within the range of 200nm-400nm.The absorbability and pKa value of sildenafil were determined at the selected wavelength of 280,287,290,295 and 297nm respectively.RESULTS： The pKa value of sildenafil was 9.35± 0.02.CONCLUSION ：The present method is convenient and practicable.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第2期134-136,共3页
China Pharmacy
关键词
紫外分光光度法
西地那非
电离平衡常数
UV Spectrophometry
Sildenafil
lonization equilibrium constant
