摘要
用悬汞电极，于Ｂｒｉｔｔｏｎ－Ｒｏｂｉｎｓｏｎ－ＣＨ３ＣＨＯ（ｐＨ９．５～１０．５）体系中研究了妥布霉素、丁胺卡那霉素等五种氨基糖苷类抗生素的吸附伏安行为，并讨论了其电极过程。认为该类抗生素结构上共有的伯、仲氨基与乙醛的衍生化反应产物，在一定电位下易吸附于电极表面。其不可逆还原峰的峰电位为－１．４５Ｖ（ｖｓ．ＳＣＥ）。在选定条件下，浓度在２．０×１０（－９）～２．５×１０（－７）ｇ／ｍＬ范围内均与还原峰峰电流呈良好的线性关系，检测限低至１．０×１０（－９）ｇ／ｍＬ。方法无需预处理即可用于软膏、尿样的测定，回收率在９０．３％～１１４％之间。
The adsorptive voltammetric behavior of five aminoglycoside antibiotics, e. g. tobramycin,amikacin, etc, has been studied with HMDE in Britton-Robinson buffer solution(pH9. 5～ 10. 5) and acetaldehyde. The electrode processes are discussed. The condensation products of the amino groups in these antibiotics with acetaldehyde can easily be adsorbed on the electrode surface under a specific potential. The peak potential of their irreversible reduction is-1. 45V(vs. SCE ) . Under optimal conditions , the peak reduction currents vary linearly with their concentrations in the range of 2. 0× 10￣(-9)～2. 5 × 10￣(-7)g/mL. Their detection limit is about 1. 0×10￣(-9)g/mL.This method can be employed satisfactorily for the determination of trace aminoglycoside antibiotics in unguent and urine samples without pretreatment. The recovery was found to be 90. 3%～114%.
出处
《分析测试学报》
CAS
CSCD
1996年第3期37-42,共6页
Journal of Instrumental Analysis
基金
浙江省自然科学基金
