摘要
二次谐波成像是近年发展起来的一种三维光学成像技术,具有非线性光学成像所特有的高空间分辨率和高成像深度,可避免双光子荧光成像中的荧光漂白效应,是一种理想的非侵入生物活体成像方法.生物组织的病变往往会引起微观结构的变化,而二次谐波信号对组织的结构对称性变化高度敏感,因此二次谐波成像对于某些疾病的早期诊断或术后治疗监测,具有很好的生物医学应用前景.介绍二次谐波成像的发展现状,生物组织中二次谐波信号的产生机理,二次谐波成像的实现手段及其在生物医学领域的应用.
Second harmonic generation imaging （SHGI） is an emerging 3-D optical imaging modality in biomedicine. Because of its inherent nonlinear mechanism, SHGI has the unique advantages of high spatial resolution, large penetration depth and non-photobleaching, and is accepted as one of the promising and non-invasive biomedical imaging modalities. Pathological changes in biological tissues usually cause changes in their microstructure. Since the second harmonic generation （SHG） signal is highly sensitive to the symmetry change of the microstructure, SHGI can be used for the early diagnosis of diseases and the monitoring of aftertreatment in clinics. This paper provides an overview of SHGI technology, signal mechanism in biological tissues, and the implementation and applications of SHGI in biomedicine.
出处
《深圳大学学报（理工版）》
EI
CAS
北大核心
2006年第1期1-9,共9页
Journal of Shenzhen University(Science and Engineering)
基金
国家自然科学基金项目(60408011
60138010)
广东省自然科学基金资助项目(5010500)
光电信息技术科学教育部重点实验室开放基金项目(2005-5)
关键词
二次谐波
非线性光学
生物组织
显微成像
早期诊断
高分辨率
活体成像
second harmonic generation
nonlinear optical imaging
biological tissue
microscopy
early diagnosis
high resolution
in vivo
