摘要
目的探讨鸡冠花黄酮化合物对去卵巢大鼠尿无机盐、骨形成蛋白(BMP2)以及对单核吞噬细胞吞噬功能的影响。方法雌性SD大鼠21只,随机分为假手术组(Sham),去卵巢组(OVX),去卵巢加鸡冠花黄酮组(cristataL,CRIST)100 mg/(kg.d),10周后测骨密度和尿液钙、钠、钾含量。免疫组化法测骨骼中BMP2的表达,单核巨噬细胞体外吞噬试验和碳廊清法测定巨噬细胞的吞噬功能。结果OVX组大鼠尿钙、钠显著增高,而尿钾及骨BMP2表达降低。CRIST组与OVX组比大鼠尿钙、钠显著降低,骨BMP2显著升高。OVX组与假手术组比大鼠单核巨噬细胞体外吞噬鸡红细胞的能力显著降低(P<0.05或<0.01);CRIST有提高单核巨噬细胞吞噬功能的作用。结论鸡冠花黄酮类化合物可调节去卵巢大鼠无机盐代谢表达,增加BMP2表达,提高巨噬细胞吞噬功能的作用,预防骨质疏松。
Objective To study the effect of eristata L flavonoid on urine mineral and the expression of bone morphogenetic protein 2 （BMP2）, and phagocytosis function of mononuelear macrophage in ovariectomized rats. Methods Twenty one female SD rats were randomly divided into three groups： Sham, ovarieetomized（OVX）, ovarieetomized with cristata L flavonoid[CRIST, 100 mg/（kg·d） ]. The content of urinary calcium, sodium, potassium were measured after ten weeks. The Streptavidin-Peroxidase Histostain^TM-Plus Kits was used to detect the expression of BMP2 in bone. And phagocytosis test of mononuclear macrophage in vitro, carbon clearance measurement were carried out to determine the phagocytotic functions of mononuclear maerophage. Results Compared with Sham group the calcium, sodium in urine expression of ovarieetomized group were higher and the potassium of urine and the expression of BMP2, and the phago percentage, and phagocytic index and the carbon clearance index were significantly decreased in OVX group（ P 〈 0.05 or 〈 0.01 ）. Compared with OVX group the urine calcium, sodium expression, and the phago-percentage, carbon phagocytic index were significantly in CRIST group, but the bone BMP2 expression was significantly higher than that of OVX group （P 〈 0.05 or 〈 0.01）. Conclusion The cristal L flavonoid could decrease the urinary calcium loss, and increased the expression of BMP2, and increased the phagocytotic functions of mononuclear macrophage. It might be advantageous to prevent the postmenopausal Osteoporosis, and to faeilitate health care in menopause women.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第2期165-166,共2页
Chinese Journal of Public Health
基金
河南省自然科学基金项目(0124170736)
