摘要
作者应用流式细胞仪技术（Ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）测定了手术切除的５４例肝细胞癌，５３例相应癌旁肝组织及９例门静脉癌栓和淋巴结转移癌组织细胞核ＤＮＡ倍体模式。探讨了癌细胞核ＤＮＡ倍性分布与肝癌临床病理特征的关系，以及癌旁肝细胞核ＤＮＡ倍性与门静脉癌栓、淋巴结转移的关系。结果显示：二倍体肿瘤主要分布于ＴＮＭ分期Ⅰ、Ⅱ期及分化较好的Ｅｄｍｏｎｄｓｏｎ分级Ⅰ、Ⅱ级肿瘤。５７％的异倍体肿瘤血清ＡＦＰ值大于４００μｇ／Ｌ，高于二倍体肿瘤（２２．２％，Ｐ＜０．０５）。伴门静脉癌栓形成、淋巴结转移、肝内转移的肝癌癌旁细胞核异倍体率明显增高。７例原发癌与其转移癌同为异倍体，２例转移癌的ＤＮＡ指数不同于其原发癌。结果揭示，同时测定癌及癌旁肝细胞核ＤＮＡ倍体模式有助于预测肝细胞癌的进展和转移。
The nuclear DNA content of resected fresh specimens in 54 carcinomas,53 corresponding adjacent liver tissues and 9 embolus in the portal vein and lymphatic metastasis of hepatocellular carcinomas(HCC) was measured by means of flow cytometry.The DNA index(DI) was calculated.The relationship between the nuclear DNA ploid pattern of carcinomas cell and clinicopathological features,and the relationship between the DNA ploidy of cancer-adjacent liver cell and portal vein cancer embolus, metaststic carcinoma of lymph nodes was discussed.The diploid tumors were mainly distributed in TNM classification Ⅰ,Ⅱand grade Ⅰ,Ⅱ well-differentiated tumor.Serum AFP levels were over 400 μg/L in 57%of the aneuploid tumors and 22.2%of the diploid tumors(P<0.05).The nuclear DNA aneuploid rate of the cancer-adjacent liver cells from HCC with embolus of the portal vein and metastasis of lymph nodesis higher, Between the metastasis and their primary tumours the 7 metastatic tumours exhibited the same DI to their primary tumors,2 cases of metastatic tumors were not the same with their primary cancer.This study indicates that the measurement of nuclear DNA ploid pattern is conductive to pridict the differentiation,progress and metastasis of HCC.
出处
《实用癌症杂志》
1996年第3期148-151,共4页
The Practical Journal of Cancer
