摘要
目的探讨抗炎性细胞因子白细胞介素(IL)10与促炎性细胞因子白细胞介素(IL)18在重症急性胰腺炎(SAP)中的表达。方法将42只大鼠按随机数字表法分为对照组和SAP组,采用3.5%牛磺胆酸钠(1mlkg)胰胆管内逆行注射诱导大鼠SAP模型。动态定量测定肺湿重系数、腹水量、血清淀粉酶、IL10、IL18的水平和以半定量RTPCR法检测肺组织中IL10mRNA与IL18mRNA的表达,并在光镜下观察胰腺及肺组织病理学改变。结果SAP组肺湿重系数、肺损伤评分、腹水量、血清淀粉酶、IL10、IL18水平和肺组织中IL10mRNA与IL18mRNA的表达与对照组相比均明显增高和增强(P<0.05),IL18与IL10水平呈负相关(r=-0.60,P<0.01)。SAP组大鼠血清中IL10与肺组织IL10mRNA水平均与肺损伤严重程度呈负相关(r=-0.66,P<0.01与r=-0.60,P<0.01),而IL18与肺组织IL18mRNA水平均与肺损伤严重程度呈正相关(r=0.74,P<0.01与r=0.79,P<0.01)。结论IL18在SAP并发肺损伤中有重要的作用,而IL10对肺损伤有保护作用。
Objective To investigate the expression of anti-inflammatory cytokine （Interleukin-10） and inflammatory cytokine （Interleukin-18） in severe acute pancreatitis （SAP）. Methods Forty-two SD rats were randomly divided into control group and SAP group. The model of SAP was established by injection of 3.5 % sodium taurochalate （ 1 ml/kg） into the bili-pancreatie duct. The levels of lung wet weight index, ascites, serum amylase, IL-10 and IL-18 were quantitatively measured at the different time point. Intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were detected by semi-quantitative RT-PCR. The histopathology of pancrease and lung were observed under the light microscope. Results The level of serum amylase, IL-10, IL-18, lung wet weight index, lung injury score, ascites, intrapulmonary expressions of IL-10 mRNA and IL-18 mRNA were significantly increased in SAP group, with statistical significance compared with the control group （P 〈 0.05）. The level of IL-18 was negatively correlated with the level of IL-10 （ r = - 0,60, P 〈 0.01）. The level of serum IL-10 and intrapulmonary expression of IL-10 mRNA are negatively correlated with the lung injury score （ r = - 0.66, P 〈 0.01 ; r = - 0.60, P 〈 0,01）. The level of serum IL-18 and intrapulmonary expression of IL-18 mRNA were positively correlated with lung injury score （r = 0.74, P 〈 0.01 ; r = 0,79, P 〈 0.01）. Conclusion IL-18 concentration may play a key role in the course of acute lung injury during severe acute panereatitis, and IL-10 exerts the protective effect in this process.
出处
《中华急诊医学杂志》
CAS
CSCD
2006年第2期107-109,共3页
Chinese Journal of Emergency Medicine
