摘要
目的探讨中药复方——糖脉平胶囊治疗2型糖尿病的作用机理。方法用较小剂量的链脲佐菌素腹腔注射(35mg/kg)及喂高热量食物造成2型糖尿病模型。造模后的动物随机分成模型组、糖脉平组和二甲双胍组,并设立正常对照组。利用放免法测定血清胰岛素水平的变化,用原位杂交法检测骨骼肌葡萄糖转运体4基因(GluT4mRNA)的表达。结果造模后,大鼠胰岛素含量明显增高,而GluT4mRNA的表达明显降低(P<0.01)。经治疗,糖脉平组胰岛素水平较治疗前明显降低(P<0.01),与正常组无区别(P>0.05),糖脉平组及二甲双胍组GluT4mRNA的表达较治疗前明显增强(P<0.01或P<0.05),胰岛素的分泌与GluT4mRNA的表达成显著的正相关(r=0.825)。结论糖脉平能改善高胰岛素血症,增加GluT4mRNA的表达,促进葡萄糖在组织中的利用,从而达到降血糖的目的,这可能是该药治疗2型糖尿病的重要机理。
Objective To investigate the mechanism of Chinese herbal medicine compound Tang Mad Ping （TMP） capsule to type 2 diabet in rats. Methods The models of type 2 diabetic rats were established by injecting lower dosage of streptozotocin （STZ, 35 mg/kg） to beUy, and fed high sucrose and high fat diet. Modle rats were randomly divided into model group, TMP group, and Erjiashuanggu （EJS） group, at the same time normal control. The content of plasma insulin was detected by radio-immunicy, mRNA expressing of frame muscle of Glucose Transporter 4 （GluT4） gene was detected with hyvirdism in situ. Results After built model, levels of insulin were obviously ascended in rats, but the expression of GluT4 mRNA lower significantly （P〈0.01）; after treatment. The levels of insulin were lower than before （P〈0.01）, and were resemble to normal content, the expressions of GluT4 mRNA TMP and EJS groups were obviously strengthen than before treatment （P〈0.01 or P〈0.05）. The secretion of insulin was related well with expression of GluT4 mRNA （r=0.825）. Conclusions TMP capsule could improve high insulin in plasma, increased expression of GluT4 mRNA, accelerated the use of Glucose in tissue, in order to descend blood glucose. It may be important mechanism that TMP capsule treat type 2 diabet.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第2期24-26,共3页
Chinese Journal of Information on Traditional Chinese Medicine
