摘要
[目的]探讨鳖甲煎丸抗肝纤维化的作用机制。[方法]采用复合因素建立肝纤维化大鼠模型,观察鳖甲煎丸对肝纤维化模型大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)等各项指标的影响及光镜下肝组织病理学变化,用免疫组化方法观察其对肝组织转化生长因子β1(TGF-β1)表达的影响。[结果]鳖甲煎丸能明显降低血清ALT、AST活性[分别为(83.13±13.21)(、197.45±23.18)U/L,与模型组比较,P<0.01],减轻模型大鼠肝组织纤维化,抑制模型大鼠肝组织TGF-β1的表达(17.23±3.81,与模型组比较,P<0.01)。[结论]鳖甲煎丸可有效防治大鼠肝纤维化,其作用机制可能是通过抑制肝纤维化组织TGF-β1的表达实现的。
[Objective] To investigate the mechanism of anti-liver fibrosis treatment by Biejia jian pill （BP）. [Methods] The liver fibrosis rat model was induced by multiple factors and was given BP. Then serum ALT, AST and pathological changes under a light microscope were observed, and the expression of transforming growth factor-beta 1 （TGF-β1） was analyzed by the method of immunohistochemistry . [Results] BP could significantly decrease serum ALT and AST activities, ameliorate the grades of liver fibrosis and inhibit the expression of TGF 61 in liver fibrosis tissue. [Conclusion]BP could effectually prevent and treat the liver fibrosis in rat model. Its mechanism could be carried out by inhibiting the expression of TGF-β1 in liver fibrosis tissue.
出处
《中国中西医结合消化杂志》
CAS
2006年第1期11-13,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
广东省中医药管理局资助课题(103085)
关键词
肝纤维化
鳖甲煎丸
转化生长因子Β1
liver fibrosis
Biejiajian pill
transforming growth factor-beta 1
