摘要
目的探讨外周血中性粒细胞及组织中肿瘤坏死因子α(TNFα)mRNA表达在创伤脓毒症中的作用。方法采用逆转录聚合酶链反应(RTPCR)检测盲肠结扎穿孔术(CLP)大鼠外周血中性粒细胞及组织TNFαmRNA的表达。结果CLP术后外周血中性粒细胞中TNFαmRNA表达逐渐增高,48h达峰值后开始降低,但仍明显高于正常对照组水平。组织TNFαmRNA表达的增高首先局限于创伤局部(小肠12hP<0.01,24h达峰值),然后进入血循环,再转移至敏感器官肺和肝组织中(肺24hP<0.01,48h达峰值;肝24hP<0.05,48h达峰值)。结论TNFαmRNA表达的增高在脓毒症发病机制中起重要作用。
Objective To evaluate the changes in tumor necrosis factor- α （TNF - α） mRNA expression in peripheral polymorphonuelear leukocyte （PMNs） and tissues after cecal ligation puncture （CLP） in rats. Methods Reverse transcription-polymerase chain reaction （RT PCR） was used to detect TNF - α mRNA expression in peripheral PMNs and tissues. Results The TNF - α mRNA expression in peripheral PMNs rose gradually after CLP, and it began to decrease after reaching the peak at 48 hours, but it was still higher than normal. The elevation of TNF- α mRNA expression was first limited in the regional tissues （P〈0.01 at 12 hours after CLP and peaked at 24 hours in the intestine）, then it entered the blood circulation later to affect the sensitive organs- lungs and livers （P〈0.0l at 24 hours after CLP and peaked at 48 hours in the lung; P〈0.05 at 24 hours after CLP and peaked at 48 hours in the liver）. Conclusion The rise of TNF-a mRNA expression plays an important role in pathogenesis of sepsis.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第1期28-31,共4页
Chinese Critical Care Medicine
基金
全军"十五"指令性课题资助项目(01L081)
