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聚乙烯亚胺对A549细胞基因转导和毒性作用研究 被引量：3

Effect of polyethylenimine on the A549 cell gene transduction and cytotoxicity
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摘要 目的探讨其聚乙烯亚胺(polyethylenimine,PEI)在转导浓度下对肿瘤细胞的毒性作用。方法以PEI为载体将增强型绿色荧光蛋白(enhanced green fluorescence protein,EGFP)报告基因转入A549肺癌细胞,应用台盼蓝染色法和四甲基偶氨唑盐(MTT)法研究不同浓度PEI下A549肺癌细胞的存活率。结果粒径86.9 nm PEI 4μg介导2μg的DNA对肺癌A549细胞的转染率最高(29%),与其余转染梯度相比有统计学差异(P<0.05);台盼蓝染色法检测和MTT法检测不同浓度PEI纳米凝胶下肺癌A549细胞的存活率与对照组间的差异无统计学意义,不同浓度间亦无差异。结论PEI是有效安全的基因转导载体。 Objective To investigate the cytoxicity effect of polyethylenimine on tumor cell under different transduction concentration. Methods A plasmid vector containing the EGFP gene was cultured with PEI of different concentration to observe the survival rate of A549 lung cancer cell. Results Cell transfection rate of A549 lung cancer cell was the highest with 4pg PEI to mediate 2pg DNA evaluated by Trypan blue staining and MTT assay; the survival rate of of A549 lung cancer cell was not significantly different between the various concentration of PEI and control group. Conclusion The PEI is a useful and safe vector for the gene transduction.
作者 姚元虎 刘永彪 冯永 韩高华
机构地区 徐州医学院肿瘤研究所
出处 《中国临床保健杂志》 CAS 2006年第1期1-3,共3页 Chinese Journal of Clinical Healthcare
基金 江苏省教育厅自然科学基金资助课题(03KJD320230)
关键词 聚乙烯亚胺 转染 毒性作用 遗传载体 肺肿瘤 Polyethylenimine Transfecfion Toxic actions Genetic vectors Lung neoplasms
分类号 R114 [医药卫生—卫生毒理学]
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参考文献13

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共引文献7

同被引文献33

  • 1姚元虎,刘永彪,冯永,韩高华.聚乙烯亚胺转导效率与肿瘤细胞周期分布相关性分析[J].中国临床保健杂志,2006,9(3):207-209. 被引量：2
  • 2姚元虎,刘永彪.肿瘤基因治疗转导载体聚乙烯亚胺[J].国际肿瘤学杂志,2006,33(7):493-496. 被引量：4
  • 3芮静安.原发性肝癌的治疗研究新进展[J].临床肝胆病杂志,2006,22(4):244-246. 被引量：11
  • 4姚元虎,冯永,冯霞,王秀英,刘永彪,徐冬梅,姚思德.聚乙烯亚胺对小鼠组织器官氧化损伤的研究[J].中国生物医学工程学报,2006,25(6):733-738. 被引量：3
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中国临床保健杂志
2006年 第1期

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