摘要
目的：从中性粒细胞与血管内皮细胞黏附的角度探讨麝香对创伤愈合的作用基础。方法：以TNF处理体外培养的人脐静脉内皮细胞（HUVEC）为模型，应用MTT法、虎红法、荧光免疫组化法研究麝香酮对人外周血中性粒细胞（PMN）与HUVEC黏附及HUVEC表面黏附分子表达的影响。结果：TNF处理HUVEC12小时，能明显增强PMN与HUVEC黏附（P〈0．01），并能明显促进HUVEC表面ICAM-1、VCAM-1和CIM4表达（P〈0．05）。75～150ml麝香酮作用于TNF活化的HU-VEC，明显抑制PMN与HUVEC黏附（P〈0．01），仅150μg/ml时降低HUVEC表面ICAM-1表达（P〈0．05），37．5μg/ml和150μg/ml时减少其表面VCAM．1表达（P〈0．05），75-150μg/ml时抑制其表面CD44表达（P〈0．05或P〈0．01）。结论：麝香酮通过降低HUVEC表面ICAM-1、VCAM-1和CD44表达而抑制中性粒细胞与血管内皮细胞黏附，可能是麝香促进慢性创面愈合的机制之一。
Objective： Based on the model of human umbilical vein endothelial cell（HUVEC） treated by tumor necrosis factoralfa （TNF）, We investigate the effects of muscone on polymophonulear leukocytes （PMN） -HUVEC adhesion and its adhesion molecules （CAMs） expression. Methods：Confocal system was used for identifying cultured HUVEC, MTT assay for its activity, Rose Bengal Staining for PMN-HUVEC adhesion, and fluorescent-immunocytochemistry techniques for CAMs expression. Results： After HUVEC treated by TNF, the adhesion between PMN and HUVEC increased dramatically（ P 〈 0. 01 ）, as well as the expressions of ICAM-1, VCAM-1 and CD44 ascended（P 〈0. 05）. Muscone markedly inhibited HUVEC adherence to PMN（P 〈 0. 01 ） by the mechanism of decreasing the expression of ICAM-1, VCAM-1 and CD44 of HUVEC （P 〈 0. 015, P 〈 0. 01, respectively）. Conclusion： Muscone inhibited PMN adherence to HUVEC triggered by TNF. The inhibitory effect is related to the decreased expression of CAMs on HUVEC. It indicated the possible pathway of musk to promote chronic wound healing by inhibiting excessive inflammation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第2期148-150,155,共4页
Chinese Journal of Immunology
基金
北京市中医药科技项目(No.JJ2003-13)
