摘要
目的:研究血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性及载脂蛋白E基因(ApoE)多态性与糖尿病视网膜病变(DR)的关系。方法:收集145例2型糖尿病患者(合并或不合并有糖尿病视网膜病变)及年龄、性别相匹配的90例正常对照组,用加入50g/LDMSO的改良PCR法检测ACE基因I/D多态性,PCR-RFLP方法检测ApoE基因型。比较各组ACE、ApoE基因型及等位基因频率分布。结果:①无视网膜病变糖尿病(NDR)组与正常对照(NC)组间ACE基因型及等位基因频率均无显著差异(P>0.05);糖尿病视网膜病变(DR)组与正常对照(NC)组相比,DD基因型频率显著升高(24.4%vs10.0%,P<0.05),D等位基因频率也显著升高(46.3%vs30.6%,P<0.01)。②NC组、NDR组及DR组3组间ApoE基因型及等位基因频率均无显著差异。结论:ACE-DD基因型及D等位基因是DR的遗传易患因子之一;ApoE基因多态性与糖尿病发病无关,并且与是否并发DR无关。
AIM： To study the relationship of angiotensin converting enzyme （ACE） and apolipoprotein E （ApoE）gene polymorphism with diabetic retinopathy.
METHODS： 145 cases of type 2 diabetes mellitus （with or without diabetic retinopathy） and 90 cases of normal controls were enrolled. The ACE genotypes were detected with improved method by adding 50g/L dimethyl sulphoxide （DMSO） in polymerase chain reaction, and the ApoE genotypes were scanned by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） respectively. ACE and ApoE genotypes and alleles frequencies were compared among three groups （NC, NDR and DR）.
RESULTS： There was no difference of ACE gene frequencies between the group of type 2 diabetesmellitus and normal controls （P〉0.05）. The frequencies of DD genotype and D allele in patients with DR （24.4%, 46.3% ） were higher than those in control subjects （10.0%, 30.6%） respectively （P〈0.05, P〈0.01）. There were no differences of ApoE genotypes and alleles frequencies among three groups.
CONCLUSION： ACE polymorphism is associated with DR, DD genotype, and D allele of ACE gene is probably one of the genetic marker of DR, ApoE polymorphism has no relationship with type 2 diabetesmellitus, nor with DR.
出处
《国际眼科杂志》
CAS
2005年第6期1156-1159,共4页
International Eye Science
