摘要
目的:构建人CXCR4原核表达载体并在大肠杆菌中进行表达。方法:从健康人外周血单个核细胞提取总RNA,以RT-PCR获得CXCR4基因全长1059bp的完整编码序列,将其克隆入载体pMD-18T中,经限制性内切酶和菌落PCR分析并测序证实的阳性重组子与表达载体pET-28a(+)连接并转化大肠杆菌BL21(DE3)。结果:12%SDS-PAGE分析表明,在30℃以IPTG诱导获得分子量为43ku的His-CXCR4融合蛋白表达带,诱导4h后此蛋白表达量约为全菌总蛋白的25%。结论:成功获得人CXCR4基因融合蛋白。
Objective：To construct prokaryotic expression vector and express human CXCR4 protein in E. coll. Methods： The total RNA was extrated from normal human PBMC and used as a template for RT - PCR. A 1059bp fragment containing the whole length gene coding region of protein CXCR4 was obtained and cloned into pMD18 - T vector. The recombinant which had analysied by restriction endonucleases and clony PCR was sequenced to confirm its idenity. The right recombinant was ligated with vector pET- 28a（ ＋ ）and transformed into E. coli BI21 （DE3）. Results： After being induced by IFTG at 30℃ for 4h,a fusion protein His- CXCR4 with an apparent MW of 43ku was obtained in 12%SDS- PAGE gel and accounted for 25% of the total cellular protein. Conclusion： Human CXCR4 fusion protein was obtained successfully.
出处
《生物技术》
CAS
CSCD
2006年第1期1-3,共3页
Biotechnology
基金
湖北省自然科学基金项目资助(No.301130921)
