摘要
目的:研究黄粉虫防御性分泌物的化学成分及其抑菌活性。方法:用二氯甲烷萃取分泌物并经GC/MS分析,后用牛津杯法和平板连续稀释法分别就该分泌物对大肠杆菌、金黄色葡萄球菌、白色假丝酵母、黑曲霉、桔青霉的抑菌作用及2-甲基对苯醌和对甲酚标准品对上述供试菌的最低抑菌浓度进行测定和比较。结果:分泌物含2-甲基对苯醌、对甲酚和正二十三烷等7种成分,抑菌强度:桔青霉>大肠杆菌和金黄色葡萄球菌>白色假丝酵母>黑曲霉。结论:该分泌物对这5种供试菌的抑制效果优于2-甲基对苯醌和对甲酚标准品。
Objective： To study on the chemical components and the antimicrobial activities of the defensive secretion of Tenebrio molitor L.. Methods： The secretion was extracted by dichlorumethane and analyzed by gas chrumatography/mass spectrometry （GC/MS）, Then Oxford Cup method was used in detecting and discussing the antimicrobial activities of the secretion against Escherichia coli, Staphylococcus aureus, Candid albicans, Aspergillus niger and Penicillium citrinum. Dilute - plate method was used to detect and discuss the minimal inhibitory concentration （MIC） of the standard materials of methyl - p - benzoquinone（MBQ） and p - cresol on the tested strains. Results： The secretion included seven kinds of chemicals, such as MBQ, p - cresol, tricosane and so forth. Its inhibitory intensity： Penicillitum citrimum 〉 Escherichia coli and Staphy- lococcus aureus 〉 Candida albicans 〉 Aspergillus niger. Conclusion： The secretion was more effective in inhibiting the growth of the five species d microbes than the standard materials of MBQ and p- cresol.
出处
《生物技术》
CAS
CSCD
2006年第1期22-24,共3页
Biotechnology
基金
福建省教育厅基金项目资助(No.JB01104)
