摘要
通过UV诱变复合酶产生菌黑曲霉筛选得到一突变株JW001,该菌株可同时发酵产生高活力的多酶系,其中纤维素酶和酸性蛋白酶活力分别为18 379 u/g和6 472 u/g,比出发菌株分别提高122%和92%.优化确定了曲盘固体发酵工艺,并进行了复合酶动物应用试验,试验表明黑曲霉JW001菌株发酵生产的饲用复合酶制剂对不同畜禽均有很好的饲喂效果.
One feed complex-enzyme producing mutant strain of Aspergillus niger JW001 was obtained by UV mutagenesis, the strain can produce high activity of multi-enzyme systems. The cellulase enzyme activity was 18 379 u/g, 122% higher than original strain and the acidic proteinase enzyme activity was 6 472 u/g, 92%higher than original strain. The solid plate fermentation process of this strain was optimized and animal feeding experiments were carried out, the results showed that the feed enzyme-complex produced by strain JW001 could bring series beneficial effects on different animals.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2006年第1期81-85,共5页
Journal of Hebei University(Natural Science Edition)
基金
河北省科学技术研究计划资助项目(95245502D)
关键词
黑曲霉
突变菌株
饲用复合酶
固体发酵
Aspergillus niger
mutant strain
feed enzyme-complex
solid fermentation.
