期刊文献+

还原型谷胱甘肽对糖尿病大鼠肾组织非酶糖基化影响的试验研究 被引量:1

Effect of reduced glutathione on nonenzymatic glycosylation in kidney of streptozotocin-induced diabetic rats.
下载PDF
导出
摘要 目的探讨还原型谷胱甘肽对糖尿病大鼠肾脏的保护作用及其可能机制。方法以链脲佐菌素诱导糖尿病大鼠模型,给予还原型谷胱甘肽、盐酸氨基胍单独或联合治疗8周,以荧光分光光度计测定肾皮质荧光值即糖基化终末产物(AGEs)含量,行肾组织PAS染色测定平均肾小球截面积(MGA)及体积(MGV),放免法测定尿白蛋白排泄率(UAER),同时检测糖化血红蛋白(HbA1C)、血清肌酐清除率(Ccr)、肾重/体重比值、血糖水平等。结果8周时,各组糖尿病大鼠Ccr、UAER、MGA、MGV、肾重/体重比值、AGEs含量均较空白对照组明显增加(P<0.05或P<0.01)。还原型谷胱苷肽、盐酸氨基胍单独或联合治疗组Ccr、UAER、MGA、MGV、肾重/体重比值、AGEs含量均较糖尿病对照组明显降低(P<0.05或P<0.01)。结论还原型谷胱苷肽及盐酸氨基胍均可减少肾组织糖基化终末产物生成,对糖尿病大鼠肾脏具保护作用。 Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-indueed diabetic rats as well as the possible mechanism involved in. Methods thods Streptozotocin-induced diabetic rats received aminoguanidine 50mgkg^-1·d^-1 and reduced glutathione 400mgkg^-1·d^-1 t intraperitoneally respectively or synchronously for 8 weeks. The fluorescence of renal cortical tissue was measured by spectrofluorometer representing contents of AGEs. Urinary albumin ex- cretion rate(UAER) was measured by radioimmunoassay. The mean glomerular area(MGA) and volume(MGV) vere measured by image analysis system. The changes of creatinine clearance rate(Ccr), the kidney welght/body weight ratio, the blood glucose and hemoglobinAlc levels were observed. Results By the end of 8 weeks, the Ccr, UAER, MGA, MGV, kidney weight/body weight ratio,and contents of AGEs increased significantly in DM groups compared with the blank control gloup (P 〈 0.05 or P 〈 0.01 ) ,and decreased significantly in aminoguanidine and reduced glutathione respectively or synchronously treatment groups compared with the DM control gloup (P 〈 0.05 or P 〈 0.01 ). Conclusion Reduced glutathione and aminoguanidine can inhibit the overproduction of AGEs in diabetic rats, and it's renoprotective mechanism varrants further investigation.
出处 《中国实验诊断学》 2006年第2期121-124,共4页 Chinese Journal of Laboratory Diagnosis
关键词 糖尿病肾病 还原型谷胱苷肽 氨基胍 非酶糖基化 diabetic nephropathy nonenzy matic glycation aminoguanidine reduced glutathione
  • 相关文献

参考文献8

  • 1Giardino I,Edelstein D,Brownlee M,et al.BCL-2 expression or antioxiants prevent hyperglycemia-induced formation of intraoellular advanoed glycation endproducts in bovine endothelial cells[J].J Clin Invest,1996,97:1422.
  • 2Soulis T,Cooper M E,Sastra S,et al.Relative contributions of advanced glycation and nitric oxide synthase inhibition to aminoguanidinemediated renoprotection in diabetic rats[J].Diabetologia,1997,40:1141.
  • 3Niwa T,Tsukushi S.3-deoxyglucosone and AGEs in uremic complications:inactivation of glutathione peroxidase by 3-deoxyglucosone[J].Kidney Int,2001 59 Suppl 78:S37.
  • 4Mizutani K,Ikeda K,Nishikata T,et al.Phytoestrogens attenuate oxidative DNA damage in vascular smooth muscle cells from strokeprone spontaneously hypertensive rats [J].J Hypertens,2000,18(12):1833.
  • 5Milchak LM,Douglas BJ.The effects of glutathione and vitamin E on iron toxicity in isolated rat hepatocytes[J].Toxicol Lett,2002,126(3):169.
  • 6Hoensch H,M orgenstern I,Petereit G,et al.Influence of clinical factots,diet,and drugs on the human upper gastrointestinal glutathione system [ J ].G ut,2002,50 (2):235.
  • 7Savoye G,Miralles BO,Dechelotte p,et al.Low levels of gastric mucosal glutathione during upper gastric bleeding associated with the use of nonsteroidal anti-inflammatorydrugs[J].Eur J Gastroenterol Hepatol,2001,13(11):1309.
  • 8Powell LA,Nally SM,M CmASTER D,et al.Restoration of glutathione levels in vascular smooth muscle cells exposed to high glucose conditions[J].Free Radic BiolMed,2001,31(10):1149.

同被引文献46

引证文献1

二级引证文献90

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部