摘要
目的研究β纤维蛋白原基因Bcl-I多态性与血浆纤维蛋白原水平及脑梗死的相关性,探讨脑梗死的遗传易感性和发病机制,为脑梗死的早期基因诊断、预防和治疗提供依据。方法应用PCR-RFLP方法对92例脑梗死组、89例健康对照组进行β-纤维蛋白原基因Bcl-I多态性分析;采用组织凝血活酶法测定血浆纤维蛋白原水平(g/L)。结果脑梗死患者血浆纤维蛋白原水平明显高于健康对照组(P<0.05),而且B1B2、B2B2基因型频率及B2等位基因频率明显增高(P<0.05);B1B2、B2B2基因型血浆纤维蛋白原水平明显高于B1B1基因型(P<0.05)。结论血浆纤维蛋白原水平升高与脑梗死有显著相关性;β纤维蛋白原基因Bcl-I多态性与血浆纤维蛋白原水平及脑梗死有相关性。提示β纤维蛋白原基因Bcl-I多态性可能是脑梗死危险因素及遗传易感标志之一。
Objective To research the association of β-fibrinogen Bcl-i polymorphisms and plasma fibrinogen levels with cerebral infarction. To explore genetic susceptible of cerebral infarction and its pathogenesis and provide evidence to early gene diagnosis, prevention and therapy. Methods Ninety-one patients with cerebral infarction and elghty-nine healthy case control were enrolled in this trail. The polymorphisms of Bel-I βfibrinogen gene was detected by polymerase chain reaction- restriction fragment length polymorphisms（PCR-RFLP）. Plasma fibrinogen levels（g/l） were assayed by tissue thromboplas- tin. Results The Plasma fibrinogen levels in patients were significantly higher than those control subjects（ P 〈 0.05）. The frequence of genotype B1 B2 and B2B2 B2 allele was higher in cases with cerebral infarction than in healthy cases control（ P 〈0.05）. The Plasma fibrinogen levels in the samples of genotype BIB2 and B2B2 was higher than that in samples of B1B1 （ P 〈0.05） .Conclusion There is significant association between the fibrinogen plasma levels and cerebral infarction, β-fibrino- gen Bcl-I gene polymorphisms are associated with plasma level of fibrinogen and cerebral infarction. These suggest that β-fib- rinogen Bcl-I gene polymorphisms maybe a risk factor and a susceptible predictor of cerebral infarction.
出处
《中国实验诊断学》
2006年第2期153-155,共3页
Chinese Journal of Laboratory Diagnosis
基金
汕头医学院李嘉诚博士后基金(2001113)
