摘要
采用分光光度法研发了一种间接测定废水中微生物产生单质硫含量的新方法。该方法是将废水中的单质硫用氯仿萃取,浓缩,用无水乙醇溶解,然后加入过量的亚硫酸氢盐,反应生成硫代硫酸盐,再与过量的碘反应,通过比色测定剩余碘量而间接测定单质硫的含量。结果表明,该方法简便易行,结果重现性好,灵敏度高,检测限为0.03mg·L-1,回收率范围在97.8%￣100.2%。采用液相色谱法对该方法进行了验证,结果显示2种方法测得的数据无显著差异。与液相色谱法相比,该法灵敏度高,所需仪器设备简单,测试费用较低。
A spectrophotometric method for indirect quantification of microorganism-produced sulfur in wastewater was developed. Sulfur in wastewater was extracted by chloroform, and then evaporated to dryness. The residue was dissolved in ethanol then treated with exceeded bisulphite to convert thiosulfate, the later was reacted with exceeded iodin and the rest was further determined by spectrophotometric method. The results revealed that the present innovative method was characterized by convenience, high sensitivity, high recovery range and well recurred, the limit of detection was as low as 0.03 mg·L^-1 and the recovery ranged from 97.8% to 100.2%. Furthermore, the spectrophotometric method was well testified by a liquid chromatographic method with no significant difference. And the innovative method was characterized by simple apparatus, high sensitivity, and low cost in comparison with the HPLC method.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2006年第1期261-265,共5页
Journal of Agro-Environment Science
基金
天津市自然科学基金资助项目"富含硫酸盐高浓度有机废水生物处理技术"(013607911)
关键词
测定
硫化物废水
单质硫
分光光度法
quantification
wastewater
sulfur
spectrophotometric method
