摘要
目的:观察颅脑损伤性肺水肿(NPE)的病理生理特点及内皮素(ET)的作用。方法:雄性SD大鼠随机分为4组:对照组10只,颅脑损伤后死亡组,颅脑损伤后6 h处死组,颅脑损伤后24 h处死组,各14只,建立弥漫性颅脑损伤动物模型;内皮素测定方法采用人内皮素-1酶免疫检测试剂盒。结果:颅脑损伤后总体死亡率为46.43%。颅脑损伤后大鼠的肺湿干比与PaO2、肺灌洗液白蛋白浓度、肺病理评分存在相关关系;肺组织匀浆ET-1与肺灌洗液白蛋白浓度及肺湿干比存在相关关系;肺灌洗液ET-1与PaO2、肺灌洗液白蛋白浓度、肺湿干比、肺病理评分存在相关关系。死亡组血浆、肺组织匀浆、肺灌洗液ET-1水平分别为(80.32±60.85)、(35.44±16.83)、(35.84±6.77)pg/mL,均明显高于其它各组,且三者之间存在相关性。结论:颅脑损伤导致NPE发生;ET-1可促进NPE的发生,其浓度水平与肺水肿的严重程度有关,其中肺组织局部ET-1起着更为重要的作用。
Objective:To observe the pathology and physiology of NPE and the function of ET. Methods: Male SD rats were divided into four groups: control group( n = 10) , dead group( n = 14) , 6 hours head injury group( n = 14), 24 hours head injury group(n = 14). We have used Assay Designs' human Endothelin-1 TiterZyme Enzyme Immunometric Assay(EIA) kit to determine the concentration of ET. Results: The mortality is 46.43%. There are linear dependences between lung wet/dry ratio and PaO2, albumin of BALF, lung pathology score. There are linear dependences between ET-1 of lung tissue homogenate and albumin of BALF, lung wet/dry ratio. There are linear dependences between ET-1 of BALF and PaO2 ,albumin of BALF, lung wet/dry ratio and pathology score. ET-1 level of blood plasma, lung tissue homogenate and BALF of Ld group are (80.32±60.85) , (35.44 ±16.83), (35.84± 6.77 ) pg/mL, which are much higher than that of other groups, and there are linear dependences between them. Conclusions: Head injury produces NPE. ET-1 can promote NPE to take place, and it's concentration is concerned with the severity of lung edema. Pulmonary ET-1 level is more important.
出处
《广州医学院学报》
2005年第5期17-21,共5页
Academic Journal of Guangzhou Medical College
基金
2004年度天坛医院青年基金课题
关键词
颅脑损伤
神经源性肺水肿
内皮素
head injury(HI)
neurogenic pulmonary edema(NPE)
endothelin(ET)