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NF-κB哑铃形诱骗剂ODN对骨髓瘤细胞生长及IL-6表达的抑制作用 被引量：3

The inhibitory effects of dumb-bell decoy oligodeoxynucleotides targeting NF-κB on the growth of multiple myeloma cells and IL-6 expression
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摘要 目的设计合成靶向NF-κB的哑铃形诱骗剂ODN,分析靶向NF-κB的哑铃形诱骗剂对NF-κB转录活性、多发性骨髓瘤细胞8266的生长及其分泌的IL-6的抑制效应。方法采用电泳迁移率变动分析(EMSA)体外检测诱骗剂ODN对NF-κB转录活性的抑制效应。将8266细胞随机分为传代培养的8266细胞组;诱骗剂ODN处理组及脂质体处理组。通过阳离子脂质体以2mg/L、4mg/L、8mg/L不同剂量诱骗剂ODN转染8266细胞。转染后8、12、18h,ELISA法检测8266细胞培养上清中IL-6的表达。用MTT比色检查诱骗剂ODN对IL-6刺激的8266细胞生长的影响。结果硫代磷酸的诱骗剂ODN在体外能有效地抑制NF-κB与其顺式元件的结合;2mg/L、4mg/L、8mg/L等不同浓度的脂质体-ODN复合物对8266细胞表达IL-6的抑制程度不同。脂质体-ODN复合物对8266细胞的生长及IL-6的活性均有抑制作用。结论靶向NF-κB的诱骗剂ODN在体外可抑制NF-κB的转录活性,从而抑制8266细胞的生长,降低瘤细胞中IL-6的表达。 AIM： To investigate the inhibitory effects of dumb-bell decoy oligodeoxynucleotides （ODNs） targeting NF-κB on the transcriptional activity of NF-κB binding to its cis-elements, growth of multiple myeloma cells 8266 and the expression of IL-6 in 8266 cells. METHODS： The inhibitory effects of dumbbell decoy ODNs targeting NF-κB on the transcriptional activity of NF-κB was detected by electrophoretic mobility shift assay （EMSA）. 8266 cells were randomly divided into 8266 cells of passage （control）, dumbbell decoy ODNs treatment, and cationic liposome treatment groups. Dumb-bell decoy ODNs of 2, 4 and 8 mg/L were cultured respectively into 8266 cells via cationic liposome vector. Then, the transfected 8266 cells and their supernatant were collected at 8, 12 and 18 h. IL-6 expression was measured by ELISA. Effect of decoy ODNs on the growth of 8266 cells stimulated with IL-6 was measured by MTT Colorimetry assay. RESULTS： Dumbbell decoy ODNs effectively inhibited the bindingof NF-κB to its cis-elements in vitro. Different doses of decoy ODNs （2, 4 and 8 mg/L） had different inhibitory effects on IL-6 expression in 8266 cells. After decoy ODNs had been transfected into 8266 cells stimulated by IL-6 via cationic liposome vector, both the growth of 8266 cells and activity of IL-6 were inhibited. CONCLUSION： Dumb-bell decoy ODNs can effectively inhibit the growth of 8266 cells and decrease the expression of IL-6 in myeloma cells through the inhibiting NF-κB activity.
作者 蒋红利 薛武军 刘华 陈葳
机构地区 西安交通大学医学院第一医院肾病中心 西安交通大学医学院第一医院分子生物学中心
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2006年第1期47-50,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 陕西省自然科学基金资助课题(No.2002G257)
关键词 NF—κB 诱骗剂ODN IL-6 多发性骨髓瘤 NF-κB dumb-bell decoy oligodeoxynucleotide IL-6 multiple myeloma cell
分类号 R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献12

  • 1Shiao RT,Meleskey SB,Khera SY,et al.Mechanisms of IL-6-mediated immunologloubin secretion by dexanethasone and suramin in human lymphoid and myeloma cell lines[J].Leuk Lymphoma,1996,21(3,4):293-297.
  • 2Ma MH,Yang HH,Parker K,et al.The proteasome inhibitor PS-341 markedly enhances sensitivity of multiple myeloma tumor cell to chemotherapeutic agents[J].Clin Cancer Res,2003,9:1136-1144.
  • 3Hongyu N,Melek E,Qin H,et al.Analysis of expression of nuclear factor κB (NF-κB) in multiple myeloma:downregulation of NF-κB induces apoptosis[J].Bri Haematol,2001,115(2):279-286.
  • 4张艰,李圣青,李焕章,戚好文,吴昌归.NF-κB在大鼠急性肺损伤模型肺组织中的表达及N-乙酰半胱氨酸的影响[J].细胞与分子免疫学杂志,2004,20(6):712-715. 被引量：19
  • 5Mitsiades N,Mitsiades CS,Richardson PG,et al.The proteasome inhibitor PS-341 potentiates sensitivity of multiple myeloma cells to conventional chemotherapeutic agents:therapeutic applications[J].Blood,2003,101(6):2377-2380.
  • 6Mann MJ,Dzau VJ.Therapeutic applications of transcription factor decoy oligonucleotides[J].J Clin Inv,2000,106(9):1071-1075.
  • 7Leblance R,Catley LP,Hideshima T,et al.Proteasome inhibitor PS-341 inhibits human myeloma cell growth in vivo and prolongs survival in a murine model[J].Cancer Res,2002,62(9):4996-5000.
  • 8Zweegman S,Huikgens PC.Treatment of myeloma:recent developments[J].Anticancer Drugs,2002,13:339-351.
  • 9Homaidan FR,Chakroun I,Esabban ME.Regulation of nuclear factor-κB in intestinal epithelial cells in a cell model of inflammation[J].Mediators Inflamm,2003,12(5):277-283.
  • 10Morishita R,Aoki M,Kaneda Y.Decoy NF-kappaB oligodeoxynucleotides as novel cardiaovascular drugs for cardiaovascular disease[J].Ann N Y Acad Sci,2001,947;294-302.

二级参考文献9

  • 1Blackwell TS, Blackwell TR, Holden EP, et al. In vivo antioxidant treatment suppresses nuclear factor-kB activation and neutrophilic lung inflammation[J]. Immuno, 1996, 157(4): 1630-1637.
  • 2Rahman I, Gilmour PS, Jimenez LA, et al. Oxidative stress and TNF-alpha induce histone acetylation and NF-κB/AP-1 activation in alveolar epithelial cells: potential mechanism in gene transcription in lung inflammation[J]. Mol Cell Biochem, 2002, 234-235(
  • 3Ward PA, Lentsch AB. Endogenous regulation of the acute inflammatory response[J]. Mol Cell Biochem, 2002, 234-235(1-2): 225-228.
  • 4Parsey Mv, kaneko D, Shender R, et al. Neutrophil apoptosis in the hung after hemorrhage or endotoxemia. Apoptoxemia; apoptosis and migration are independen of IL-1[J]. Clin Immunol, 1999, 91: 219-225.
  • 5Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NF-κB and preseroation of IkB2 by interleukin-10 and inter leubin-13[J]. J Clin Invest, 1997, 100(10): 2443-2448.
  • 6Lentsch AB, Czermak BI, Bless NM, et al. NF-κB activation during IgG immune complex-induced lung injury[J]. Am J Pathol, 1998, 1 52(5): 1327-1336.
  • 7Parsey Mv, Tuder R, Abraham E. Neutrophils are major contributors to intraparethymal lung IL-lexpression after hemorrhage and endotoxemi-α[J]. J Immunol, 1998, 160: 1007-1013.
  • 8Beg AA, Sha WC, Bronson RT, et al. Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-κB[J]. Nature, 1995, 376: 167-170.
  • 9Sha WC, Liou HC, Tuomanen E, et al. Targeted disruption of the p50 subunit of NF-κB leads to multifocal defects in immune responses[J]. Cell, 1995, 80: 321-330.

共引文献18

同被引文献35

  • 1郭益群,陈世伦.多发性骨髓瘤骨髓基质细胞分泌IGF-1、VEGF和IL-6的动态变化[J].中华血液学杂志,2006,27(4):231-234. 被引量：12
  • 2李勇华,侯健,王东星,傅卫军,袁振刚,陈玉宝.含沙利度胺方案治疗多发性骨髓瘤11O例临床分析[J].国际输血及血液学杂志,2007,30(4):300-304. 被引量：5
  • 3SEN R,BALTIMORE D. Multiple nuclear factors interacts with the immunoglobulinenhanee sequences [ J ]. Cell, 1986,46 ( 5 ) : 705 - 716.
  • 4T RACEY L, PEREZ2RO SADO A, A RTIGA M J, et al. Expression of the NF-kappaB targets BCL 2 and BIRC50 Survivin characterizes small B-cell and aggressive B-cell lymphomas,respectively [J]. JPatho l,2005,206 (2) :123-134.
  • 5WATANABE M, DEWAN MZ, OKAMURA T,et al. A novel NF- kappaB inhibitor DHMEQ selectively targets constitutive NF-kappaB activity and induces apoptosis of multiple myeloma ceils in vitro and in vivo[ J]. Int J Cancer,2005,114( 1 ) :32 -38.
  • 6XIAOW, HODGEDR, WANGL,et al. NF-kappaB activates IL-6 expression Through cooperation with c-Jun and IL6-AP1 site, but is independent of its IL6-NfkappaB regulatory site in autocrine human multiple myeloma cells[ J]. CancerBioTher,2004,3 (10) :1007 - 1017.
  • 7SILVA S, KOVALCHUK AL, KIM JS,et al. BCL2 accelerates inflammation-induced BALB/c plasmacytomas and promotes novel tumors with coexisting T ( 12 ; 15 ) and T ( 6 ; 15 ) translocations [ J ]. Cancer Res,2003,63 ( 24 ) : 8656 - 8663.
  • 8ALEXANDRAKIS MG, PASSAM FH, GANOTAKIS ES, et al. The clinical and prognostic significance of erythrocyte sedimentation rate (ESR) ,serum interleukin-6 (IL-6) and acute phase protein levels in multiple myeloma [ J ]. Clin lab Haematol,2003,25 ( 1 ) : 41 -46.
  • 9JOURDAN M,MOREAUX J, VOS JD,et al. Targeting NF-kappaB pathway with an IKK2 inhibitor induces inhibition of multiple myeloma cell growth [ J 1. Br J Haematol,2007,138 (2) : 160 - 168.
  • 10Spanti D, Marcantoni C, Di Landro D. The kidney in dysproteinemia.G Ital Nefrol 2005,22(33):39 -45.

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细胞与分子免疫学杂志
2006年 第1期

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