摘要
目的采用免疫组化标记人血白蛋白微泡造影剂，观察其在兔肝脏VX2肿瘤造影实质相中的病理分布，目的是解释肝肿瘤超声造影显影的病理机制。方法10只荷VX2肝肿瘤新西兰白兔，经耳缘静脉团注人血白蛋白氟碳微泡造影剂，剂量0．04ml／kg；造影剂推注前设定为0时间点，静脉团注后分别在30s、3min、5min、10min时间点快速取肝脏组织及肿瘤组织各2～3块。用鼠抗人HAS-11抗体对标本进行免疫组化染色，SP法分析造影剂在肝和VX2肿瘤组织中的分布。结果观测时间点内，检测样本中VX2肿瘤组织偶见散在的造影剂免疫阳性反应复合物表达，而造影剂免疫阳性复合物在肝组织内分布的顺序和表达程度不同：注射后30s，仅肝细胞有散在的阳性表达，注射后3～10min，阳性反应产物在肝细胞中逐渐增多、增强，而在肝窦内的阳性棕黄色反应颗粒在窦壁表达逐渐增多；团注后造影剂的免疫反应在汇管区则以3min、5min时表达最为明显，10min时几乎无阳性表达。结论人血白蛋白微泡在肝细胞、肝窦内、窦壁以及汇管区周围均有不同程度的分布，其在肝内分布随着时间延长有从窦内向窦壁、肝细胞转移的倾向。
Objective To provide the evidence for the enhanced imaging of tumor by ultrasound contrast agent by observing the pathology during enhanced imaging in parenchymal phase. Methods 10 New Zealand rabbits bearing hepatic VX2 tumors were intravenously administered perfluorocarbon filled human albumin microbubbles through ear veins at 0.04 ml/kg when the livers were surgically exposed. The liver and VX2 tumor sample strips were quickly removed at baseline contrast and 30 s, 3 min, 5 min, 10 min after contrast injection. Then the samples were stained using HAS - 11 monoclonal Anti - Human Serum Albumin （ mouse lgG2a isetype） and were examined under a light microscope. Results Under specific anti - human albumin stain, 30 s after contrast injection, scattered positive expression was only found in hepatocyte. From 3 min to 10 min, positive stained particles increased and accumulated at hepatocyte, hepatic sinuseid space, hepatic sinus. The portal area was found significant positive at 3 - 5 min, but was almost negative at 10 min. Conclusion Human albumin positive expression was found in hepatocyte, hepatic sinusoid space, hepatic sinus, and pipeportal area, with a trend from sinus to hepatocyte.
出处
《临床超声医学杂志》
2006年第1期6-8,共3页
Journal of Clinical Ultrasound in Medicine
基金
国家自然基金项目(30200265
30470468)
关键词
超声检查
造影剂
肝肿瘤
免疫组织化学
Ultrasound
Contrast agent
Hepatic VX2 tumor
Immunohistoehemistry
