摘要
背景:骨组织工程材料的研究是组织工程研究中最有成就的领域之一,但人工骨的合成机制研究尚有许多问题不清楚。目的:探讨人工骨合成过程中胶原与磷酸钙的作用机制。设计:单一样本实验。单位:红河学院材料研究室。材料:实验于2003-07/08在红河学院材料研究室完成。胶原(10g/L醋酸溶液);氯化钙、磷酸二氢钠、氢氧化钠、Tris、盐酸等;去离子水。方法:采用液氮冻结-冷冻干燥法,制备胶原-磷酸钙复合物A,B,以及矿化过程中不同时间的状态样品;用紫外分光光度计测定胶原-磷酸钙生物矿化动力学曲线,根据曲线规律,确定制备电镜样品时的不同取样时间;根据电镜照片和能谱数据进行机制分析。主要观察指标:胶原-磷酸钙复合物的形貌及其结构随时间的变化规律。结果:①在搅拌条件下,通过缓慢中和法生成的胶原-磷酸钙复合物A为束状或针状。②在静态条件下进行生物矿化,形成的胶原-磷酸钙等复合物B在矿化初期为层状结构,与纯胶原的自组装结构相似,碳、氧、磷、钙4种元素的摩尔比依次为:7.26∶20∶0∶2;矿化末期的结构呈致密的条状,有一定的纹理,有很细的沟纹,碳、氧、磷、钙4种元素的摩尔比依次为:11.02∶22.5∶1.06∶2。结论:胶原生物矿化早期,胶原首先与钙离子配位,并按胶原自组装方式形成载钙的层状胶原模板,然后磷酸根在已成形的模板上同钙离子生成磷酸钙;通过控制搅拌条件和作用时间,能够获得到网状结构和针状结构的胶原复合物材料。
BACKGROUND： Study on bone tissue-engineered material is one of the most successful fields＇in tissue engineering, but the mechanism on synthesis of artificial bone has not been known in many aspects. OBJECTIVE： To explore the mechanism of collagen and calcium phosphate （CP） in artificial bone synthesis. DESIGN： Single sample experiment was designed. SETTING： Material Research Room of Honghe University. MATERIALS： The experiment was performed in Material Research Room of Honghe University from July to August 2003. The materials included collagen （10 g/L acetic acid solution）, calcium chloride, sodium dihydrogen phosphate （SDP）, sodium hydroxide （NaOH）, Tris, hydrochloric acid and deionized water （DI water）. METHODS： Liquid nitrogen freezing and freeze-drying were used to prepare collagen-CP complexes A and B and the samples at different times during mineralization. UV spectrophotometer was used to determine the biomineralized dynamic eurve of collagen-CP. Based on law of curve, the different times of sample collection were determined in preparation of electronic microscopic samples. According to electronic microscopic pictures and spectral data, mechanism analysis was carried MAIN OUTCOME MEASURES： Morphology of collagen-CP complex and law of its structure with time change RESULTS： ①Under agitation, collagen-CP complex A was sheaf-like or needle-like in structure manufactured with retarded neutralization. ②Under static state, with biomineralization, collagen-CP complex B was in layered structure at initial phase of mineralization, which was similar to the self-assembled structure of pure collagen and the molar ratio of C, O, P and Ca was 7.26： 20： 0： 2. At the end of mineralization, the structure was strip-like in high density with a certain grains and very fine rills and the molar ratio of C, O, P and Ca was 11.02： 22.5： 1.06： 2. CONCLUSION： At the early phase of biomineralization, collagen is coordinated initially with calcium ion, calcium-carrier layered collagen template is formed with the self-assembling of collagen, and then phosphates is combined with calcium ion to manufacture calcium phosphate in the formed template. By controlling agitation and acting time, collagen complex material of reticular and spinal structure is obtained.
出处
《中国临床康复》
CSCD
北大核心
2006年第1期172-174,i0008,共4页
Chinese Journal of Clinical Rehabilitation
