摘要
目的比较非肽类血管紧张素Ⅱ受体Ⅰ型阻断剂氯沙坦(Losartan)和血管活性肽降钙素基因相关肽(CGRP)对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖作用的影响,为探讨此两类物质的降压机制提供实验依据。方法采用MTT,3H-参入法和流式细胞仪分别测定血管紧张素Ⅱ刺激下,Losartan或CGRP干预下血管平滑肌细胞的增殖变化,W est-ern b loting法测定不同状态下血管平滑肌细胞内ERK1/2的活性变化。结果Losartan或CGRP能抑制血管紧张素Ⅱ刺激下血管平滑肌细胞的生存率、DNA合成、细胞周期增殖指数,以及细胞内ERK1/2的活性,并呈剂量依赖性。而且,CGRP抑制作用强于Losartan。结论Losartan或CGRP能抑制血管紧张素Ⅱ刺激下血管平滑肌细胞增殖,其细胞内的信号传导途径与ERK1/2有关。
Aim To compare the effects of the nonpeptide angiotensin Ⅱ receptor type Ⅰ antagonist, Losartan, and the active vascular peptide, calcitonin gene-related peptide （CGRP） , on the proliferation of vascular smooth muscle cells induced by angiotensin Ⅱ , and to explore the mechanism of depressor effect of Losartan and CGRP in vivo. Methods MTT, [^3H]- Thymidine incorporation and flow cytometry, were used to determine the ability of proliferation of VSMC induced by angiotensin Ⅱ in the presence or absence of Losartan or CGRP, Western blotting was used to determine the activity of ERK1/2. Results Losartan or CGRP inhibited the viability, DNA synthesis, cell proliferation index, and the activity of ERK1/2 in a dosedependent manner. Conclusion Losartan or CGRP significantly inhibits the proliferation of VSMC induced by angiotensin Ⅱ; the inhibitory effect of CGRP is stronger than that of Losartan. The signaling path way is involved in ERK1/2.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第1期101-105,共5页
Chinese Pharmacological Bulletin
基金
国家重点基础研究发展计划(973规划)资助项目(NoG2000056905)
