摘要
[目的]探讨不同节段项韧带钙化与脊髓型颈椎病黄韧带退变的相关性。[方法]取脊髓型颈椎病患者黄韧带192块(项韧带钙化28个节段,相应黄韧带标本56块)和青壮年颈椎创伤(非颈椎病)的黄韧带标本38块。对切除的黄韧带标本进行测量及组织学检查;用W oessner法测定其胶原含量;用盐析法对黄韧带中的Ⅰ型/Ⅱ型胶原含量比进行测定;对黄韧带厚度与羟脯氨酸、胶原干粉含量及Ⅰ型/Ⅱ型胶原含量比进行相关性研究。[结论]颈椎病组中项韧带钙化相应节段的黄韧带弹力纤维含量下降,胶原纤维含量增加,弹力纤维发生排列紊乱。实验组中C4、5、C5、6项韧带钙化相应的节段黄韧带厚度、羟脯氨酸含量、Ⅰ型/Ⅱ型胶原含量比较阴性对照组有显著差异(P<0.05);而C3、4、C6、7项韧带钙化相应节段的黄韧带上述指标则无显著差异(P>0.05)。[结论]C4、5、C5、6节段的项韧带钙化与颈椎病黄韧带的退变有相关性;上述节段的项韧带钙化提示存在应力异常分布,可能会加剧黄韧带退变,进而影响脊髓型颈椎病的发病进程。
[ Objective ] To study the relationship between ossification of ligamentum nuchae （LN） and degeneration of ligamentum flavon （LF） of cervical spondylotic myelopathy （CSM） . [ Method ] Fifty-six cervical LF samples from CSM patient of C3-7 in relation to the stage of ossification of LN samples （experimental group, EG） and 38 samples of corresponding ligament from patient with cervical vertebral trauma （ CVT ） （ control group, CG ） were obtained. Thickness of LF was measured, their pathological change was examined by microscopy, content of collagen and kydrolyproline and the ratio of type Ⅰ / type Ⅱ collagen were determined by Woessner＇s method and setting Salting out method. The correlation among the thickness of degenerative LF, the ratio of type Ⅰ/ type Ⅱ collagen, the content of collagen and hydrokyproline, and arrangement disorder of LF in relation to the ossification stage of LN were analyzed. [ Result] In EG, as compared with in CG, there showed a decrease of elastic fibre, decrease of ratio type Ⅰ / type Ⅱ collagen due to significant increase of type Ⅱcollagen, increase collagen content and disorder arrangement of LF. In relation to the ossification of LN, above changes were more marked in LF of C4.5, C5.6, than that of C3.4, C6.7 （ P 〈 0. 05 ）. [ Conclusion] The degeneration of LF is correlated with ossification stage of C4.5 , C5.6LN, The ossification of LN, that resulted in abnormal distribute of stress in LF, may be a main etiologic factor to CSM.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第3期203-205,共3页
Orthopedic Journal of China
关键词
项韧带
钙化
黄韧带
退变
相关性
Ligamentum flavon
Ligamentum nuchae
Degeneration
Ossification
Cervical spondylotic myelopathy
