摘要
目的:研究在核因子κB受体活化因子配基(RANKL)和巨噬细胞集落刺激因子(M-CSF)联合应用诱导体外骨髓细胞培养系统中,转化生长因子(TGF-β)对破骨细胞分化的影响。方法:选用小鼠M-CSF依赖性非附着性骨髓细胞,在含有25 ng/m l sM-CSF和30 ng/m l sRANKL的α-MEM培养液中进行培养,比较加入和不加入1 ng/m lTGF-β1培养4、9 d后,所形成的抗酒石酸酸性磷酸酶染色(TRAP)阳性多核细胞的数目和骨吸收面积。结果:小鼠骨髓细胞在RANKL和M-CSF共同作用下可形成TRAP阳性多核巨细胞,并具有骨吸收功能。加入TGF-β后,破骨样细胞的数目及骨吸收面积显著增加。结论:TGF-β对RANKL和M-CSF诱导的破骨细胞的分化及其功能的促进作用,可能为炎症状态下发生过度骨吸收的机制之一。
Objective:To investigate the effect of TGF-β on osteoclast differentiation in primary murine bone marrow cell culture in the presence of receptor activator of NF-κB Ligand (RANKL) and macrophage colony stimulating facotr(M-CSF). Methods:M-CSF-dependent bone marrow cells were isolated from 5 -6 weeks old mice, and cultured in the presence of RANKL at 30 ng/ml and M-CSF at 25 ng/ml with or without I ng/ml of TGF-β for 4 and 9 days respectively. TRAP positive multinucleated cells and resorption pits on dentine slices were counted under light microscope. Results:TRAP positive multinucleated cells were induced from murine bone marrow cell cultures with both RANKL and M-CSF, and these cells formed resorption pits on dentine slices. The number of TRAP positive multinucleated cells and resorption pits were significantly increased by 1 ng/ml of TGF-β( P 〈0. 01 ). Conclusion: TGF-β can augment osteoclast formation and function, which may partially account for the bone destruction under inflammation.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第1期38-40,共3页
Journal of Practical Stomatology
关键词
巨噬细胞集落刺激因子
破骨细胞
骨髓细胞
转化生长因子Β
细胞分化
Receptor activator of NF-κB ligand
Macrophage colony stimulating factor
Transforming growth factor-β
Osteoclast
Bone marrow cells