摘要
以反刍动物为研究对象,应用多重PCR技术扩增绵羊基因组中X、Y染色体上的4个微卫星标记和SRY基因,根据基因型进行性别鉴定,试图通过一次DNA扩增同时提供性别鉴定和基因分型的信息。结果表明所设计SRY基因的引物具有高度特异性,是性别鉴定的主要依据,而Y染色体上的MCM158、MAF45两标记由于特异性不好,因此不适用于性别鉴定,对于X染色体上所选的两标记MILVET09和AE25只能进一步验证所鉴定的雄性个体。得出结论在被检个体中,能同时扩增出SRY基因、MCM158、MAF45,X染色体上MILVET09和AE25,且X染色体上的MILVET09、AE25基因型为纯合子的个体为正常的雄性;被检个体中只有Y染色体上MCM158、MAF45和X染色体上MILVET09、AE25的扩增产物,而没有SRY基因的扩增产物,则被检个体为雌性,且MILVET09、AE25的基因型对雌性个体的性别判断无影响。MCM158、MAF45两标记基因型不影响个体的性别鉴定结果。
Multiplex PCR amplification of four microsatellite markers on chromosomes X and Y and the SRY gene was used to identify the sex and genotype the ruminant simultaneously in one step. Result indicated that the d SRY primers was highly specific and could be used as the main basis for sex-identification. But primer specificity was not good for Y chromosome markers MCM158 and MAF45, and therefore these markers were not suited for sex identification. The primers of two X chromosome markers MILVET09 and AE25 are amplified, but the SRY gene is not, then the individual being examined is female. Furthermore the genotype of MILVET09 and AE25 dose not affect sex determination of the female. The genotype of markers MCM158 and MAF45 dose not affect the result of sex-identification, only were able to further confirm the male identity. In conclusion： when SRY, MCM158 and MAF45 on chromosome Y and MILVET09 and AE25 on chrosomome X can all be amplified simultaneously, and when the genotype of MILVET09 and AE25 is hornozygous, the animal is determined to be normal male~ When only MCM158, MAF45 and MILVET09 and AE25 are amplified, but the SRY gene is not, then the individual being examined is female. Furthermore the genotype of MILVET09 and AE25 does not affect sex determination of the female. The genotype of markers MCM158 and MAF45 dose not affect the result of sex-identification.
出处
《遗传》
CAS
CSCD
北大核心
2006年第2期133-138,共6页
Hereditas（Beijing)
基金
德国联邦科技与教育部(BMBF)资助(项目代号:CHN00316)~~
关键词
SRY基因
微卫星标记
性别鉴定
SRY gene
microsatellite marker
sex-identification
