摘要
目的研究人乳头状瘤病毒(HPV)E6、E7作用位点的各种细胞周期蛋白在宫颈癌的表达情况。方法选择73例HPV16阳性的宫颈鳞癌癌组织标本,其中35例来自澳大利亚,38例来自中国。采用半定量的免疫组化方法检测p53、pRb、p16INK4A、p21CIP1/WAF1、p27KIP1和细胞周期素D1(cyclin D1)的表达情况。结果两组患者在平均年龄、淋巴结转移、肿瘤细胞分化程度方面的差异无统计学意义,但中国组较澳大利亚组进展期宫颈癌(>Ⅱa期)患者为多(P=0.007)。澳大利亚组宫颈癌组织p53的阳性表达率及pRb、p21和p27的上调率分别为3%、56%、63%和34%,中国组分别为23%、89%、97%和89%,两组癌组织中上述四种细胞周期蛋白的表达情况差异有统计学意义。p16和cyclin D1的上调率在澳大利亚组分别为97%和3%,在中国组分别为和100%和12%,两组比较差异无统计学意义。两组资料合并,p53总的阳性表达率为13%,pRb、p16、p21、p27和cyclinD1总的上调率分别为74%、99%、81%、63%和7%。结论HPV16阳性的宫颈癌明显存在pRb、p16、p21和p27的过度表达,而中国组宫颈癌p53阳性表达率和pRb、p21、p27的过度表达率比澳大利亚组明显增高,这提示HPV诱导宫颈癌的分子学途径复杂,有必要在不同人种进行进一步研究。
Objective To investigate the patterns of expression of key cell cycle proteins targeting on the human papillomavirus （ HPV ） sites E6 and E7 in cervical carcinoma. Methods Semiquantitative immunohistochemistry was used to determine the expression of the cell cycle regulatory factor p53, retinoblastoma gene product pRb, cyclin-dependent kinase inhibitors p21^CIP1/WAF1 （ p21 ） , pl6^INK4A （ p21 ）, and p27^KIP1 （ p21 ）, and cyclin D1 targeting on the HPV sites E6 and E7 in 73 HPV 16-positive specimens of cervical squamous cell carcinoma, 35 from Australia patients and 38 age, lymph node status, and grade of tumor-matched Chinese patients. Results There were no significant differences in age, lymph node status, and grade of tumor between the Chinese and Australian groups, however, the number of the patients in advanced stage （ 〉Stage IIa） was greater in the Chinese group than in the Australian group （19：7）. The positive rate of p53 in the Australian group was 3% , significantly lower than that in the Chinese group （23% , P =0.028）. The upregulation rate of pRb, p21, and p27 in the Australian group were 56% , 63% and 34% respectively, all significantly higher than those in the Chinese group （ 89% , 97% , and 89% respectively, P = 0. 01, P 〈 0. 001, and P 〈 0. 001 ）. There were no significant differences in the upregulation rate of p16 and cyclin D1 expression rate between the Australian group and Chinese group （97% versus 100%, and 3% versus 12%, both P 〉0.05）. In the combined data of both groups, the positive rate of p53 was 13%; and the upregulation rates of pRb, pl6, p21, p27, and cyclin D1 were 74% , 99%, 81%, 63% and 7% respectively. Conclusion Cervical carcinoma overexpresses pRb, pl6, p21, and p27. Tumors from Chinese patients are significantly more likely to be positive in p53 and to have upregulated pRb, p21, and p27 than their Australian counterparts. The molecular pathways to human papillomavirus-induced cervical cancer may be influenced by ethnicity and further investigation is necessary.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第5期312-316,共5页
National Medical Journal of China
关键词
乳头状瘤病毒
人
宫颈肿瘤
蛋白
细胞周期
Papillomavirus, human
Cervix neoplasms
Egg white
Cell cycle
