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Changes of learning, memory and levels of CaMKII, CaM mRNA, CREB mRNA in the hippocampus of chronic multiple-stressed rats 被引量:21

Changes of learning, memory and levels of CaMKII, CaM mRNA, CREB mRNA in the hippocampus of chronic multiple-stressed rats
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摘要 Background The effect of chronic stress on cognitive functions has been one of the hot topic in neuroscience. But there has been much controversy over its mechanism. Such single stressor applied in the past could not simulate complicated living circumstances that people confronted with. The aim of this study was to investigate the effects of chronic multiple-stress on learning and memory as well as on the levels of calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ), calmodulin (CAM) mRNA, and cAMP-response element binding protein (CREB) mRNA in the hippocampus of rats. Methods The rats were divided randomly into stressed and control groups. The stressed group was given chronic multiple-stress for 6 weeks to set up a chronic multiple-stressed model. The rats' performance of spatial learning and memory was tested using Morris Water Maze (MWM) and Y-maze. Meanwhile, the expressions of CAMKII, CAM mRNA and CREB mRNA of rats' hippocampus were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction (RT-PCR), respectively. In addition, the width of synaptic cleft and the thickness of post-synaptic densities (PSD) were observed in the hippocampal CA3 region of rats by electron microscopy. Results After exposure to chronic multiple-stress for 6 weeks, the ability of learning and memory of the stressed group was higher than that of the control group (P〈0.05, P〈0.01). The width of synaptic cleft was smaller and the thickness of PSD was larger in the hippocampal CA3 region of the stressed group than in that of the control group (P〈0.01). The CAMKII immunostaining of the stressed group was stronger than that of the control group in the stratum radiatum and oriens of the hippocampal CA1 and CA3, especially in the stratum oriens. Quantitative analysis indicated that the expression of CAMKII, CAM mRNA, and CREB mRNA in the hippocampus of the stressed group was higher than that of the control group (P〈0.05, P〈0.01). Conclusions The capacity of learning and memory can be enhanced after chronic multiple-stress. The increased levels of CAMKⅡ, CAM mRNA, and CREB mRNA may contribute to the enhancing effect of chronic multiple-stress on learning and memory. Background The effect of chronic stress on cognitive functions has been one of the hot topic in neuroscience. But there has been much controversy over its mechanism. Such single stressor applied in the past could not simulate complicated living circumstances that people confronted with. The aim of this study was to investigate the effects of chronic multiple-stress on learning and memory as well as on the levels of calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ), calmodulin (CAM) mRNA, and cAMP-response element binding protein (CREB) mRNA in the hippocampus of rats. Methods The rats were divided randomly into stressed and control groups. The stressed group was given chronic multiple-stress for 6 weeks to set up a chronic multiple-stressed model. The rats' performance of spatial learning and memory was tested using Morris Water Maze (MWM) and Y-maze. Meanwhile, the expressions of CAMKII, CAM mRNA and CREB mRNA of rats' hippocampus were detected by immunohistochemistry, Western blot and reverse transcription-polymerase chain reaction (RT-PCR), respectively. In addition, the width of synaptic cleft and the thickness of post-synaptic densities (PSD) were observed in the hippocampal CA3 region of rats by electron microscopy. Results After exposure to chronic multiple-stress for 6 weeks, the ability of learning and memory of the stressed group was higher than that of the control group (P〈0.05, P〈0.01). The width of synaptic cleft was smaller and the thickness of PSD was larger in the hippocampal CA3 region of the stressed group than in that of the control group (P〈0.01). The CAMKII immunostaining of the stressed group was stronger than that of the control group in the stratum radiatum and oriens of the hippocampal CA1 and CA3, especially in the stratum oriens. Quantitative analysis indicated that the expression of CAMKII, CAM mRNA, and CREB mRNA in the hippocampus of the stressed group was higher than that of the control group (P〈0.05, P〈0.01). Conclusions The capacity of learning and memory can be enhanced after chronic multiple-stress. The increased levels of CAMKⅡ, CAM mRNA, and CREB mRNA may contribute to the enhancing effect of chronic multiple-stress on learning and memory.
出处 《Chinese Medical Journal》 SCIE CAS CSCD 2006年第2期140-147,共8页 中华医学杂志(英文版)
基金 This study was supported by a grant from the National Natural Science Foundation of China (No. 30270446).
关键词 LEARNING MEMORY calcium/calmodulin-dependent protein kinase calmodulin mRNA cAMP-response element binding protein mRNA learning memory calcium/calmodulin-dependent protein kinase Ⅱ calmodulin mRNA cAMP-response element binding protein mRNA
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  • 1孙臣友,刘能保,张敏海,刘少纯,程少荣,李辉,刘向前,汪薇曦.慢性复合性应激大鼠学习和记忆与海马突触后致密物质CaMKII的表达变化[J].解剖学报,2005,36(1):19-23. 被引量:17
  • 2刘能保,李辉,刘向前,孙臣友,陈少容,张敏海,刘少纯,汪薇曦.慢性复合应激增强大鼠空间学习和记忆能力[J].生理学报,2004,56(5):615-619. 被引量:37
  • 3Lupien S J,de Leon M,de Santi S,et al.Cortisol levels during human aging predict hippocampal atrophy and memory deficits[].Nature Neuroscience.1998
  • 4Gallagher M,Rapp PR.The acute effects of corticoid on cognition: integration of animal and human model studies[].Brain Research Brain Research Reviews.1997
  • 5Ohl F,Fuchs E.Differential effects of chronic stress on memory processes in the tree shrew[].Brain Research Cognitive Brain Research.1999
  • 6Bowman R E,Zrull M C,Luine V N.Chronic restraint stress enhances radial arm maze performance in female rats[].Brain Research.2001
  • 7Conrad CD,LeDoux JE,Magarinos AM,et al.Repeated restraint stress facilitates fear conditioning independently of causing hippocampal CA3 dendritic atrophy[].Behavioral Neuroscience.1999
  • 8Morris R.Development of a water-maze procedure for studying spatial learning in the rat[].Journal of Neuroscience Methods.1984
  • 9Chen Z,Zhao Q,Sugimoto Y,et al.Effects of histamine on MK-801-induced memory deficits in radial maze performance in rats[].Brain Research.1999
  • 10Bennecib M,Gong CX,Grundke-Iqbal I,et al.Role of protein phosphatase-2A and -1 in the regulation of GSK-3, cdk5 and cdc2 and the phosphorylation of tau in rat forebrain[].FEBS Letters.2000

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