摘要
目的化学法去除同种异体神经髓鞘和雪旺细胞,形成去细胞基膜管支架后复合甲状腺激素,来桥接大鼠坐骨神经缺损,研究神经再生效果。方法SD大鼠坐骨神经用化学法处理得到去细胞基膜管支架;外源性三碘甲状腺原氨酸(T3)经化学处理后形成无菌、pH值中性的T3水溶液然后溶解在明胶水凝胶中,局部注射在去细胞基膜管支架上。实验分3组:去细胞基膜管复合甲状腺激素移植组(A)、去细胞基膜管移植组(B)和自体神经移植组(C)。术后进行形态学、坐骨神经功能指数(SFI)、神经电生理学、光镜、电镜及图像分析检测。结果术后8周SFI比较,C组(-36.36±1.91)优于A组(-41.58±2.98)和B组(-41.89±3.65),差异有统计学意义(P<0.01),A组和B组间差异无统计学意义(P>0.05)。术后12周,A组(-31.38±1.87)和C组(-30.03±1.73)优于B组(-36.74±1.63),差异有统计学意义(P<0.01),A组和C组间差异无统计学意义(P>0.05)。运动神经传导速度(NCV)、远端再生有髓神经截面积恢复率在8周、12周两时间点上,A组、C组均优于B组,差异有统计学意义(P<0.01),A组和C组差异无统计学意义(P>0.05)。结论复合甲状腺激素的去细胞基膜管修复神经缺损,效果优于单纯去细胞基膜管移植,与自体神经移植相比较,是一种很好的自体神经移植替代方法。
Objective To study role of acellular allogeneic grafts （removed of the allogeneic nerve myelin sheath and Schwann cells by chemical method） combined with triiodothyronine （T3 ） in bridging 1.0 cm long sciatic nerve gap and observe the regenerative effect of the sciatic nerves. Methods Acellular nerve segments containing Schwann cell basal lamina tubes were obtained from sciat- ic nerves of normal SD rats via chemical extraction. T3 solution for 500 μl （0.5 mg of T3 was dissolved in 0.5 ml 0.01 N NaOH and then the solution was neutralized with 0.01 N HCl, with final PH of 7.8） was impregnated in 0. 5 ml gelatin hydrogels （0.3 g of gelatin was dissolved in 2 ml distilled water）. The SD rats were divided randomly into three Groups （10 rats in each group） ： Group A （acellular basal lamina tubes grafting combined with T3 ） , Group B （allogeneic nerve grafting） and Group C （autogenous nerve grafting）. The sciatic nerve function index （SFI） , electrophysiological test, light and electron microscopic and image analysis were done postoperatively. Results At the 8th week postoperatively, SFI was - 36.36 ± 1.91 in Group C, higher than - 41.58 ± 2.98 in Group A and - 41. 89 ± 3.65 in Group B, with significantly statistical difference （P 〈0. 01 ） but insignificantly statistical difference between Group A and Group B （P 〉 0.05）. However, at the 12th week postoperatively, SFI was - 31.38 ± 1.87 in Group A and - 30.03 ± 1.73 in Group C, both higher than - 36.74 ± 1.63 in Group B, with significantly statistical difference （P 〈 0. 01 ）, but with insignificantly statistical difference between Group A and Group C （ P 〉 0. 05 ）. Motor nerve conductive velocity （NCV） and recovery rate of the cross area of myelinated nerve fibers in both Groups A and C were significantly better than those in Group B at the 8th and 12th weeks respectively postoperatively, with significantly statistical difference （P 〈 0. 01 ）, but insignificantly statistical difference between Group A and Group C （ P 〉 0. 05 ）. Conclusions The acellular basal lamina tubes grafting combined with T3 is better than the acellular basal lamina tubes grafting in improving nerve regeneration and is a good substitute for nerve grafts.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2006年第2期140-143,共4页
Chinese Journal of Trauma
关键词
周围神经
化学萃取
甲状腺激素
Peripheral nerves
Chemical extraction
Thyroid hormones
