摘要
目的研究成釉细胞瘤细胞在无血清培养基中的生长特性,建立成釉细胞瘤细胞的无血清培养法。方法用DefinedKeratinocyte-SFM(DK-SFM)无血清培养基和DMEM培养成釉细胞瘤细胞,观察细胞的生长特性、细胞形态和传代次数,流式细胞仪(FCM)测定S期细胞比率(SPF)和增殖指数(PI)。结果在DK-SFM培养基中,细胞传代6次,平均生长92d;细胞形态清晰,仅见少许散在的成纤维细胞生长;SPF值为12.3%～14.5%,PI值为11.6%～15.3%。在DMEM中,细胞传代3次,平均生长61d;细胞境界不清,并可见较多的成纤维细胞;SPF值为7.9%～9.2%,PI值为8.3%～9.6%。结论成釉细胞瘤细胞在DK-SFM无血清培养基中存活时间长,DK-SFM较DMEM更适合体外培养成釉细胞瘤细胞。
[Objective] To investigate the growth behavior of ameloblastoma ceils cultured in serum-free medium and to establish the serum-free culture method for ameloblastoma cells in vitro. [Methods] The Defined Keratinocyte-SFM （DK-SFM） medium and DMEM medium were used for ameloblastoma cell culture in vitro. The growth rates in different culture media were observed. The morphology and passages of ameloblastoma ceils in different media were investigated. The SPF value and PI value of the ceils were detected by flow cytometry. [Results] In DK-SFM medium, ameloblastoma ceils were taken through 6 passages and maintained about 92 days. The shape of cultured ceils in DK-SFM medium was distinct. Only some sporadic fibroblast was seen in DK-SFM medium. The SPF value and PI value of ceils cultured in DK-SFM were 12.3%±14.5% and 11.6%±15.3%, respectively. However, in DMEM medium, the ceils were only taken through 3 passages and maintained about 61 days, and the shape of it was not clear. A lot of fibroblasts were seen in DMEM medium. The SPF value and PI value of ceils cultured in DMEM were 7.9%±9.2% and 8.3%±9.6%, respectively. [Conclusion] Ameloblastuma ceils have long survival time in DK-SFM medium, which is better for the growth of ameloblastuma ceils than that of DMEM medium.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第1期113-116,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金资助项目(30471896)
广东省自然科学基金资助项目(04300240)
关键词
成釉细胞瘤
培养基
细胞培养
ameloblastoma
culture medium
cell culture
